Accession PRJNA800007
Title RNAseq analysis of Hep3B cells upon overexpression of LINE1 retrotransposon encoded protein L1orf1p
Relevance Medical
Data types Transcriptome or Gene expression
Sample scope Multiisolate
Organism Homo sapiens [Taxonomy ID: 9606]
Description L1 retrotransposons are known to get activated during hepatocarcinogenesis. We have developed inducible (TET-ON) overexpression of L1orf1 using piggybac vector in Hep3B cell line and a control TET-ON-empty cell line was also generated. Influence of L1orf1 overexpression on overall cellular transcritome was then analysed by RNAseq followed by pathway analysis. Overall, a number of key oncogeneic pathways are dysregulated upon L1orf1 overexpression is observed. Overall design: RNAseq was carried out for 2 cell lines derived from Hep3B cells (TET-ON-L1orf1 and TET-On-empty control) 72 hrs after doxycycline (1ug/ml) induction (RNA isolated from 3 independent cell pellets).
Publication
PubMed ID Article title Journal name DOI Year
36707636
External link
Link description
Organization Applied Biosciences, Northumbria University
Data Source NCBI

Project Data

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Experiment (6)  show -