| HRA000365
(Controlled Access)
|
Multi-omics analysis of hearts provides new insights into cardiac physiology and pathology. The transcriptome and epigenome characteristics of the mammalian heart are insufficiently revealed. Here, we applied the nucleosome occupancy and methylome sequencing (NOMe-seq), which detected DNA methylation and chromatin accessibility simultaneously, as well as RNA-seq, for multi-omics analysis of four chambers of both human and mouse adult hearts, as well as human fetal hearts. We identified differentially expressed genes between the atrium and the ventricle of human and mouse adult hearts, revealing both species-conserved and specific genes, particularly a number of novel lncRNAs. Genes associated with cardiac diseases showed chamber-specific expression patterns. DNA methylation distinguished between the atrium and the ventricle in both adult and fetal hearts, and human fetal and adult hearts showed distinct chromatin accessibility profiling. |
