| Accession | SAMC1676118 |
|---|---|
| Accession in Other Database | GSA-Human: HRS168674 |
| Sample name | JZ10 |
| Title | in-vivo formed CD90+ spermatogonia cells isolated from OA patients (NO.2) |
| Sample type | Human sample |
| Organism | Homo sapiens |
| Description | obstructive azoospermia patients CD90+ spermatogonia cells |
| Attributes | *Only part of the sample information is displayed on this page because the related dataset HRA000848 has not yet been registered in the Human Genetic Resource Management Platform of MOST. |
| Release date | 2021-05-31 |
| BioProject Accession | PRJCA004680 |
| Submitter | Yuan Wang (ywang@bio.ecnu.edu.cn) |
| Organization | East China Normal University |
| Submission date | 2023-06-18 |
| Resource name | Description |
|---|---|
| GSA-Human (1) | - |
| HRA000848 (Open Access) | In this study, we developed a feeder-, serum-, and animal product-free culture condition, and successfully induced high percentage of SLCs from human PSCs. Genome-wide expression analyses demonstrated a significant upregulation of germ cell specific genes in SLCs derived from PSCs compared to in vivo isolated human CD90+ SSCs. And furthermore, under this optimized feeder-free condition SLCs have went through meiosis and formed putative round spermatids. Our data demonstrated a robust feeder-, serum- and xeno-free protocol to induce differentiation of PSCs to SLCs from which putative haploid spermatids may develop. |