样本编号 SAMC1896254
外部数据库编号 GSA-Human: HRS278504
样品名称 S5-C-O-rep3
样本标题 tumor derived organoid
样品类型 Human sample
物种名称 Homo sapiens
描述信息 single cell RNA-seq
样本属性 *该样本包含更多受控访问信息,请通过GSA-Human系统申请项目HRA001252数据获取。
发布日期 2023-01-01
项目编号 PRJCA002751
提交者 Rui  Wang  (tangfuchou@pku.edu.cn)
提交单位 Peking University
提交日期 2023-06-18

样本包含数据信息

资源名称 描述
GSA-Human (1) -
HRA001252  (Controlled Access) We adopted single-cell RNA-Seq method to analyze 8,085 cells from matching adjacent normal tissues, primary tumors, and metastatic tumors of 11 metastatic colorectal cancer patients. Furthermore, for two of the patients, we combined single-cell RNA sequencing and single-cell point mutation identification by cDNA Sanger sequencing, which permit us to identify the important phenotypic differences between the cancer cells with and without critical point mutations in the same patient in vivo at single-cell resolution. The PPAR signaling pathway was activated in tumors, and inhibiting it could drastically inhibit the growth of CRC organoids in vitro. Notably, distinct origins of lymph nodes and liver metastases were revealed in the same patients, which were verified by both CNVs and mitochondrial mutation profiles at single-cell resolution. Our study elucidates molecular heterogeneity of CRC as well as potential therapeutic target for CRC therapy.