Accession |
SAMC200958 |
Sample name |
BC1 |
Title |
Black control of rhizosphere microbiome of potato |
Sample type |
Metagenome or environmental sample |
Organism |
Bacteria |
Description |
The total DNA was extracted using improved CTAB method and purified using a DNA fast purifying kit. The V4 - V5 hypervariable region of the 16S rRNA gene was amplified using the 515F and 909R primers according to previous reports. PCR production was purified using a SanPrep gel DNA extraction kit. All purified PCR productions were pooled together with an equal molar amount from each sample and sequencing using MiSeq system. |
Attributes |
Isolation source | rhizosphere |
Collection date | 2018-04-06 |
Geographic location | China: Huizhou |
Latitude and longitude | 23.05 N 114.47 E |
Reference for biomaterial | |
Relationship to oxygen | aerobe |
Sample collection device or method | |
Sample material processing | |
Sample size | |
source material identifiers | |
|
Release date |
2020-07-22 |
BioProject Accession |
PRJCA003098 |
Submitter |
Lutian Mao (nijiajia2005@126.com)
|
Organization |
Huizhou University |
Submission date |
2020-07-22 |