| HRA001948
(Controlled Access)
|
By performing single-cell RNA sequencing (scRNA-seq) of tumor-infiltrating immune cells (TIICs) from colorectal cancer (CRC) patients, we found that macrophages and granulocytic myeloid-derived suppressor cells (gMDSCs) displaying the highest immune-inhibitory signatures were the most increased immunocytes in tumors compared to corresponding normal tissue. Tumor-infiltrating myeloid cells exhibited significantly increased expression of ITIM-bearing receptors such as SIRPA, CLEC4A and SIGLEC9. Intriguingly, Sirpa-/- mice but not Clec4a2-/- or Siglece-/- mice were more resistant to tumor progression than wild-type (WT) mice. These findings suggest that Sirpa deletion enhances innate and adaptive immune activation in the TIME; thus, Sirpa blockade could be a promising strategy to improve cancer immunotherapy. |
