样本编号	SAMC2201751
外部数据库编号	GSA-Human: HRS405129
样品名称	KO-USP7-1#-RNA-2
样本标题	KO-USP7-RNA-seq
样品类型	Human sample
物种名称	Homo sapiens
描述信息	RNA-seq data of Knock out USP7 in H1 hESC
样本属性	*该样本包含更多受控访问信息，请通过GSA-Human系统申请项目HRA002669数据获取。
发布日期	2024-07-16
项目编号	PRJCA010549
提交者	Guangjin Pan (pan_guangjin@gibh.ac.cn)
提交单位	Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
提交日期	2023-06-18

样本包含数据信息

资源名称	描述
GSA-Human (1)	-
HRA002669 (Controlled Access)	Here, we reveal that USP7, a deubiquitinase, directly modulates mTOR activity, the key driver of protein synthesis, in human embryonic stem cells (hESCs) and is essential for hESC function. USP7 directly modulates mTOR activity through K63-linked polyubiquitination. USP7-/- hESCs exhibit elevated ribosome assembly and protein synthesis, which impairs their fate specification into the three embryonic germ layers. Furthermore, we reveal an immediate downregulation of K63-linked polyubiquitination and protein synthesis prior to transcriptional changes at early differentiation onset in hESCs. Finally, we demonstrate the essential role of USP7 in regulating the translational switch during pluripotency exit. In summary, we identify USP7 and its noncanonical K63-linked polyubiquitination as a novel cell-intrinsic translational control mechanism essential for stem cell function.

资源名称

描述

GSA-Human (1)

HRA002669 (Controlled Access)

Here, we reveal that USP7, a deubiquitinase, directly modulates mTOR activity, the key driver of protein synthesis, in human embryonic stem cells (hESCs) and is essential for hESC function. USP7 directly modulates mTOR activity through K63-linked polyubiquitination. USP7-/- hESCs exhibit elevated ribosome assembly and protein synthesis, which impairs their fate specification into the three embryonic germ layers. Furthermore, we reveal an immediate downregulation of K63-linked polyubiquitination and protein synthesis prior to transcriptional changes at early differentiation onset in hESCs. Finally, we demonstrate the essential role of USP7 in regulating the translational switch during pluripotency exit. In summary, we identify USP7 and its noncanonical K63-linked polyubiquitination as a novel cell-intrinsic translational control mechanism essential for stem cell function.