样本编号 SAMC2520655
外部数据库编号 GSA-Human: HRS545027
样品名称 HEK-293T-Mork-r3
样本标题 Control for RNA editing
样品类型 Human sample
物种名称 Homo sapiens
描述信息 Replicate 3 of blank control
样本属性
分离株名称 not collected
年龄
实验材料提供者 Zefeng Wang's Lab, SINH, 320 Yueyang Road, Shanghai, China
性别 not applicable
组织器官 embryonic kidney
疾病名称
细胞系
细胞亚型
细胞类型
收集/保存方法
发育阶段
疾病分期
血统性(民族)
健康状况
核型
表型
人口
种族(人种)
类型
处理方法
用户自定义属性
发布日期 2023-01-20
项目编号 PRJCA014279
提交者 zefeng  wang  (wangzefeng@picb.ac.cn)
提交单位 Shanghai Institute of Nutrition and Health, CAS
提交日期 2023-06-18

样本包含数据信息

资源名称 描述
GSA-Human (1) -
HRA003839  (Open Access) Programmable base editing of RNA enables rewriting the genetic codes on specific sites. Current tools for specific RNA editing dependent on the assembly or recruitment of the guide RNA into an RNA/protein complex, which may cause delivery barrier and low editing efficiency. Here we report a new set of tools, RNA editing with individual RNA-binding enzyme (REWIRE), to perform precise base editing with a single engineered protein. The REWIRE system contains a human-originated programmable RNA-binding domain (PUF domain) to specifically recognize target sequence and different deaminase domains to achieve A-to-I or C-to-U editing. By utilizing this system, we have achieved editing efficiencies up to 80% in A-to-I editing and 65% in C-to-U editing, with a few non-specific editing sites in the targeted region and a low level off-target effect globally.