Accession | SAMC3006270 |
---|---|
Accession in Other Database | GSA-Human: HRS905106 |
Sample name | 20A041551 |
Title | Hepatocellular Carcinoma |
Sample type | Human sample |
Organism | Homo sapiens |
Description | DNA methylation profiling to determine the primary sites of metastatic cancers using formalin-fixed paraffin-embedded tissues |
Attributes | *This sample record contains additional controlled-access information that is avaiable from GSA-Human by requesting study HRA005166 in the GSA-Human system. |
Release date | 2023-08-17 |
BioProject Accession | PRJCA018404 |
Submitter | Shutao He (jtshi@sibcb.ac.cn) |
Organization | Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences |
Submission date | 2023-08-18 |
Resource name | Description |
---|---|
GSA-Human (1) | - |
HRA005166 (Controlled Access) | Identifying the primary site of metastatic cancer is critical to guiding the subsequent treatment. Approximately 3-9% of metastatic patients are diagnosed with cancer of unknown primary sites (CUP) even after a comprehensive diagnostic workup. However, a widely accepted molecular test is still not available. Here, we report a new method that applies formalin-fixed, paraffin-embedded tissues to construct reduced representation bisulfite sequencing libraries (FFPE-RRBS). We then generate and systematically evaluate 28 molecular classifiers, built on four DNA methylation scoring methods and seven machine learning approaches, using the RRBS library dataset of 498 fresh-frozen tumor tissues from primary cancer patients. |