| 描述信息 |
Nuclei were isolated from brain tissue as previously described. Initially, the mouse forebrain was precisely sectioned into sub-2 mm pieces and homogenized in an ice-cold buffer using a Wheaton Dounce Tissue Grinder. The homogenization process comprised 15-20 strokes with the A pestle, followed by 5-10 strokes with the B pestle. Subsequently, homogenates were passed through a cell strainer to collect the nuclear fraction. The isolated nuclear fraction underwent density gradient separation using iodixanol solutions, and nuclei were subsequently collected from the 30%-33% iodixanol interface after centrifugation. For further purification, nuclei were resuspended in a nuclear wash and resuspension buffer, and debris was removed through a cell strainer. |
