| HRA006382
(Controlled Access)
|
Epstein-Barr virus (EBV) infects 95% of adults worldwide. In healthy carriers, EBV reactivation is generally short without clinical relevance. In contrast, latent infection with sporadic lytic activation is found in undifferentiated nasopharyngeal carcinoma (NPC). In the present study, we integrated RNA sequencing data of EBV transcriptomes in NPC tissues and purified soluble antigens in prokaryotic system targeting EBV genes with high transcripts in tumors for probing serological characteristics and diagnostic performance of NPC seromarkers. Through serological profiling of EBV antibodies, we established a novel strategy combining EBV nuclear antigen 1 (EBNA1)-IgA and BNLF2b-IgG for NPC detection, which were both independent of NPC progression, indicating that latent infection is the key to the onset of NPC. |
