| HRA006742
(Controlled Access)
|
Primary cultures of NP-derived fibroblasts were stimulated with 100 ng/ml of human recombinant GZMK for 6 hours, with the culture medium used as the control. Total RNA was extracted from cultured fibroblasts. The RNA purification, reverse transcription, library construction, and sequencing steps were performed by Shanghai Majorbio Bio-pharm Biotechnology Co., Ltd. (Shanghai, China).After quantified by Qubit 4.0, the paired-end RNA-seq sequencing library was subsequently sequenced using the NovaSeq 6000 sequencer (Illumina) with a read length of 2 * 150 bp. |
