| GVM000702 | VCF sample testing is performed, followed by the construction of a GBS library. The constructed GBS library is subjected to library testing, and after passing the library testing, IlluminaHiseqPE150 sequencing is performed. The original sequence data is obtained, which is called RawData. Then, the interference information in the original sequence data is removed, and the effective data obtained is called CleanData. Count the number of Reads captured by NlallI Ecorl at both ends of clean reads, and the ratio of the number of Reads captured to the number of clean reads, i.e. enzyme capture rate, to evaluate enzyme digestion efficiency. The construction of the reference genome was carried out using sample CMI2 for Slack clustering and the reference sequence was constructed. Use software such as SAMTOOLS to detect population SNP in samples. SAMTOOLS software detects common SNP sites, filters them, and uses high-quality SNP sites for subsequent analysis. |
