| HRA006901
(Open Access)
|
To characterize the transcriptome landscape of patients with PRP, the full-thickness skin tissues were collected and fixed in 10% buffered formalin, followed by embedding in paraffin wax, from six patients with PRP carrying KRT32 mutations and six age-, gender-, and site-matched healthy controls. The paraffin-embedded tissues were sectioned at a thickness of 10 micron using a rotary microtome. FFPE tissue sections were processed for RNA isolation using Deparaffinization Reagent and CAT5 Reagent. The NEBNext PolyA mRNA Magnetic Isolation Module and NEBNext Ultra II mRNA Library Prep Kit for Illumina were used for mRNA isolation and library construction following the manufacturers protocols. Finally, library molar concentration was determined using the KAPA Library Quant kit (illumina) universal qPCR Mix. And high-throughput transcriptome sequencing was performed on an Illumina NovaSeq6000 platform according to the instructions of manufacturer. |
