Accession SAMC393778
Sample name DDX5+/+ mESCs Rep #1 (miRNA-seq)
Title DDX5+/+ mESCs Rep #1 (miRNA-seq)
Sample type Model organism or animal sample
Organism Mus musculus
Description Wild-type and DDX5 knockout mESCs were cultured on feeders-coated plates in DMEM high-glucose media containing 15% FBS, 1mM sodium pyruvate, 1mM non-essential amino acids,GlutaMAX, 0.1 mM 2-mercaptoethanol, 1000 U/ml leukemia inhibitory factor (LIF) (Millipore), and the 2i inhibitors, 3 mM CHIR99021 (Selleck), and 1 mM PD0325901.
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Biomaterial provider Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
Sex male
Tissue Cell culture
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Release date 2021-06-18
BioProject Accession PRJCA005419
Submitter Hongjie  Yao  (yao_hongjie@gibh.ac.cn)
Organization Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences
Submission date 2021-06-18

Sample Data

Resource name Description
GSA (1) -
CRA004391 RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions
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