Accession SAMC394370
Sample name ESCs rep1_RNA-seq
Title ESCs rep1_RNA-seq
Sample type Model organism or animal sample
Organism Mus musculus
Description Mouse ESCs, 4F-iPSCs and CiPSCs were maintained on feeder layers of mitomycin C-treated MEFs in an ESC culture medium containing DMEM high-glucose media containing 15% FBS, 1mM sodium pyruvate, 1mM non-essential amino acids, GlutaMAX, 0.1 mM 2-mercaptoethanol, 1000 U/ml leukemia inhibitory factor (LIF) (Millipore), and the 2i inhibitors, 3 mM CHIR99021 (Selleck), and 1 mM PD0325901.The feeder layers were removed on gelatin-coated plates before collecting cells.
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Biomaterial provider Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
Sex male
Tissue Cell culture
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Release date 2021-06-18
BioProject Accession PRJCA005419
Submitter Hongjie  Yao  (yao_hongjie@gibh.ac.cn)
Organization Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences
Submission date 2021-06-20

Sample Data

Resource name Description
GSA (1) -
CRA004402 Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs
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