| Accession |
SAMC394400 |
| Sample name |
OSKM-RNaseH1-D7-DRIPseq-rep1 |
| Title |
OSKM-RNaseH1-D7-DRIPseq-rep1 |
| Sample type |
Model organism or animal sample |
| Organism |
Mus musculus |
| Description |
MEFs at passage 2 were plated in a 12-well plate and then infected twice with retroviral stocks generated with Plat-E cells. 3 volumes of OSK supernatants and 1/4 volume of M transcription factor were mixed with 1 volume of fresh MEF medium containing polybrene. The infection efficiency was tested by MEFs transduced with virus of pMXs-EGFP. pMXs-RNaseH1 and RNaseH1(D209N) were also transfected into OG2-MEFs by retrovirus generated from PlatE cells. For infecting OG2-MEFs, the medium of OG2-MEFs was replaced with 2.125mL of infection mixture per well in a 12-well plate. Infected cells were cultured with iCD1 medium or mESC medium containing Vitamin C post infection and renewed daily. |
| Attributes |
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Breed
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Strain
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Age
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Biomaterial provider
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Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
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Sex
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not applicable
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Tissue
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Cell culture
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Birth date
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Birth location
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Breed history
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Breed method
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Cell line
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Cell type
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Collected by
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Culture collection
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Death date
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Disease
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Disease stage
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Geographic location
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Growth protocol
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Health state
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Isolation source
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Latitude and longitude
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Phenotype
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Type
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Specimen voucher
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Storage conditions
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Stud book number
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Treatment
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| Release date |
2021-06-30 |
| BioProject Accession |
PRJCA005419 |
| Submitter |
Hongjie Yao (yao_hongjie@gibh.ac.cn)
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| Organization |
Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences |
| Submission date |
2021-06-20 |
