| 样本编号 |
SAMC394442 |
| 样品名称 |
OSKM-Day6-RNAseq-rep1 |
| 样本标题 |
OSKM-Day6-RNAseq-rep1 |
| 样品类型 |
Model organism or animal sample |
| 物种名称 |
Mus musculus |
| 描述信息 |
MEFs at passage 2 were plated in a 12-well plate and then infected twice with retroviral stocks generated with Plat-E cells. 3 volumes of OSK supernatants and 1/4 volume of M transcription factor were mixed with 1 volume of fresh MEF medium containing polybrene. The infection efficiency was tested by MEFs transduced with virus of pMXs-EGFP. pMXs-RNaseH1 and RNaseH1(D209N) were also transfected into OG2-MEFs by retrovirus generated from PlatE cells. For infecting OG2-MEFs, the medium of OG2-MEFs was replaced with 2.125mL of infection mixture per well in a 12-well plate. Infected cells were cultured with iCD1 medium or mESC medium containing Vitamin C post infection and renewed daily. |
| 样本属性 |
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品种
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年龄
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实验材料提供者
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Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
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性别
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not applicable
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组织器官
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Cell culture
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出生时间
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出生地点
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品种历史
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饲养方法
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细胞系
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细胞亚型
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细胞类型
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采样单位
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收集/保存方法
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死亡日期
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发育阶段
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疾病名称
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疾病分期
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基因型
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地理分布
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养殖方法
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健康状况
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取样来源
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经纬度
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表型
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类型
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凭证标本
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贮藏条件
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记录册编号
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处理方法
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中医症候
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| 发布日期 |
2021-06-30 |
| 项目编号 |
PRJCA005419 |
| 提交者 |
Hongjie Yao (yao_hongjie@gzlab.ac.cn)
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| 提交单位 |
Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences |
| 提交日期 |
2021-06-20 |
