样本编号	SAMC4392996
外部数据库编号	GSA-Human: HRS1408673
样品名称	h2-P18-2
样本标题	h2-P18-2
样品类型	Human sample
物种名称	Homo sapiens
描述信息	To explore the gene expression profile of cell pluripotency
样本属性	*由于样本关联的数据集HRA009039尚未在科技部完成人类遗传资源信息备案，本页面只展示部分样本信息。
发布日期	2024-11-20
项目编号	PRJCA031305
提交者	tianqing Li (litq@lpbr.cn)
提交单位	Institute of Primate Translational Medicine, Kunming University of Science and Technology
提交日期	2024-11-21

样本包含数据信息

资源名称	描述
GSA-Human (1)	-
HRA009039 (Open Access)	Extraembryonic mesoderm cells (EXMCs) are involved in the development of multiple embryonic lineages and umbilical cord formation, where they subsequently develop into mesenchymal stem cells (MSCs). The present report described a three-dimensional differentiation protocol to induce hpESCs into EXMCs by activating the Wnt pathway using CHIR99021. Single-cell transcriptome and immunostaining analyses revealed that the EXMC characteristics were similar to those of post-implantation embryonic EXMCs. Cell sorting was used to purify and expand the EXMCs. Importantly, these EXMCs secreted extracellular matrix proteins, including COL3A1, and differentiated into MSCs. Inconsistent with other MSC types, these MSCs exhibited a strong differentiation potential for chondrogenic and osteogenic cells and lacked adipocyte differentiation. Together, these findings provided a protocol to generate EXMCs and subsequent MSCs from hpESCs.

资源名称

描述

GSA-Human (1)

HRA009039 (Open Access)

Extraembryonic mesoderm cells (EXMCs) are involved in the development of multiple embryonic lineages and umbilical cord formation, where they subsequently develop into mesenchymal stem cells (MSCs). The present report described a three-dimensional differentiation protocol to induce hpESCs into EXMCs by activating the Wnt pathway using CHIR99021. Single-cell transcriptome and immunostaining analyses revealed that the EXMC characteristics were similar to those of post-implantation embryonic EXMCs. Cell sorting was used to purify and expand the EXMCs. Importantly, these EXMCs secreted extracellular matrix proteins, including COL3A1, and differentiated into MSCs. Inconsistent with other MSC types, these MSCs exhibited a strong differentiation potential for chondrogenic and osteogenic cells and lacked adipocyte differentiation. Together, these findings provided a protocol to generate EXMCs and subsequent MSCs from hpESCs.