| Accession |
SAMC477384 |
| Sample name |
Hira.rescue.1-476.H2A.Z.rep2 |
| Title |
rep2 of H2A.Z ChIP-seq of R1 cells with Hira knock out and transfected vector containing Hira truncation 1-476 |
| Sample type |
Model organism or animal sample |
| Organism |
Mus musculus |
| Description |
The stable cell lines of Hira truncation overexpression were generated from Hira-KO cell line. After 48h transfection with pCAG-Flag of target protein or empty vector, cells underwent the resistance selection for a week. We check the protein level of exogenous overexpressed proteins by western blot before performing ChIP-seq and ChIP-qPCR. Cells were first crosslinked by formaldehyde, then sonicated in SDS containing buffer to dissolve the chromatin. For anti-H2A.Z ChIP-seq assay, the clarified chromatin were immunoprecipitated with Protein A/G dyna beads (Invitrogen 10002D, 10004D) conjucated with specific antibody. For anti-HA ChIP-seq assay, the clarified chromatin were immunoprecipitated with HA agarose resin (Sigma, A2095).The ChIP-seq antibody is anti-H2A.Z (Abcam, ab4174). |
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Strain
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Age
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2 Month(s)
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Biomaterial provider
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Institute of Biophysics
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Sex
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male
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Tissue
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R1 cell
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| Release date |
2021-11-05 |
| BioProject Accession |
PRJCA004722 |
| Submitter |
Liwei Zhang (lwzhanghz@ibp.ac.cn)
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| Organization |
Institute of Biophysics, Chinese Academy of Sciences |
| Submission date |
2021-11-05 |
