| HRA010716
(Open Access)
|
We demonstrate that the modulation of BMP, WNT, and Nodal signaling pathways can rapidly and efficiently induce the differentiation of both naive and primed hESCs into ExM-like cells (ExMs), predominantly through intermediates exhibiting a primitive streak (PS)-like phenotype. We reveal the regulatory roles of WNT and Nodal signaling in ExM specification from hESCs. Furthermore, we find that the initial pluripotent state influences hESC-based ExM specification, including signal response, cellular composition, developmental progression, and transcriptional characteristics of the resulting ExMs. |
