| HRA012531
(Controlled Access)
|
This study used high-resolution spatial multi-omics technology to draw a "panoramic picture" of organogenesis at the critical stage of human embryonic CS12. After slicing along the sagittal plane, six groups of adjacent tissues were subjected to HE staining, spatial transcriptome sequencing, and spatial metabolic mass spectrometry to achieve the integration of dual-modal data at the same point. The transcriptional regulation of 17 cell/tissue groups was analyzed by precise alignment using a graphical algorithm, combined with RNA rate, cell communication, and transcription factor regulatory networks; subgroups such as hepatocytes, cardiomyocytes, and skeletal muscle precursors were further subdivided to reveal the trajectory of developmental differentiation. Differential metabolism and gene-metabolism coupling analysis elucidate the energy and signal reprogramming in organ formation, providing key resources and frameworks for the study of innate developmental mechanisms and the causes of malformations. |
