| 样本编号 |
SAMC6215201 |
| 样品名称 |
RNAseq_DKO_DN_ rep1 |
| 样本标题 |
RNAseq of DN ILC3s from Utxflox/floxJmjd3flox/flox Rorccre mice rep1, female intestine |
| 样品类型 |
Model organism or animal sample |
| 物种名称 |
Mus musculus |
| 描述信息 |
Small intestinal ILC3 subsets were sorted from siLP of Utxflox/floxJmjd3flox/flox Rorccre mice for RNAseq. Limited RNA (more than 200pg, high-quality) was extracted and amplified with oligo-dT and dNTPs.incubated at 72'C and immediately put back on ice, then reverse transcribed to cDNA based onpolyA tail. The template was switched to the 5' end of the RNA and the full-length cDNA wasgenerated by PCR. Agilent 2100 bioanalyzer instrument (Thermo Fisher Scientific, MA. USA)was used to determine the average molecule length of PCR product. Purified cDNA from previoussteps was fragmented into small pieces with fragment buffer by PCR, and the product was purifiedand selected by the Agencourt AMPure XP-Medium kit (Thermo Fisher Scientific, USA). |
| 样本属性 |
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品种
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C57BCL6
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种
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Utxflox/floxJmjd3flox/floxRorccre
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年龄
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6 Week(s)
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实验材料提供者
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School of Medicine, Zhejiang University
|
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性别
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female
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组织器官
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small intestine
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出生时间
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出生地点
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品种历史
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饲养方法
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细胞系
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细胞亚型
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细胞类型
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采样单位
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收集/保存方法
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死亡日期
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发育阶段
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疾病名称
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疾病分期
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基因型
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地理分布
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养殖方法
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健康状况
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取样来源
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经纬度
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表型
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类型
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凭证标本
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贮藏条件
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记录册编号
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处理方法
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中医症候
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| 发布日期 |
2025-12-11 |
| 项目编号 |
PRJCA050649 |
| 提交者 |
Xianzhi Gao (11971006@zju.edu.cn)
|
| 提交单位 |
Zhejiang University |
| 提交日期 |
2025-12-07 |
