Characterizing noise structure in single-cell RNA-seq distinguishes genuine from technical stochastic allelic expression

We sequence mRNA from single mESCs from three culture conditions: serum + LIF, 2i + LIF and alternative 2i + LIF. We extensively analysed population and single cell gene expression to identify differences and similarities between conditions.

RNA-seq of coding RNA, co-expression, growth condition design, in vitro

Single cell mRNA -sequencing reveals cell-to-cell variation in three mouse ES cell culture conditions