DEG,DEG,DEG,DEG,DEG,DEG,DEG,DEG,DEG,DEG,DEG,10,,update,database,essential,genes,includes,protein-coding,genes,noncoding,genomic,elements,combination,high-density,transposon-mediated,mutagenesis,high-throughput,sequencing,led,significant,advancements,research,essential,genes,,resulting,dramatic,increase,number,identified,prokaryotic,essential,genes,diverse,conditions,revised,essential-gene,concept,includes,essential,genomic,elements,,rather,focusing,protein-coding,genes,DEG,10,,new,release,Database,Essential,Genes,,available,http://www.essentialgene.org,,,developed,accommodate,quantitative,qualitative,advancements,addition,increasing,number,bacterial,archaeal,essential,genes,determined,genome-wide,gene,essentiality,screens,,DEG,10,also,harbors,essential,noncoding,RNAs,,promoters,,regulatory,sequences,replication,origins,essential,genomic,elements,determined,vitro,,also,vivo,,diverse,conditions,including,survival,,pathogenesis,antibiotic,resistance,developed,customizable,BLAST,tools,allow,users,perform,species-,experiment-specific,BLAST,searches,single,gene,,list,genes,,annotated,unannotated,genomes,Therefore,,DEG,10,includes,essential,genomic,elements,different,conditions,three,domains,life,,customizable,BLAST,tools,DEG:,database,essential,genes,Essential,genes,genes,indispensable,support,cellular,life,genes,constitute,minimal,gene,set,required,living,cell,constructed,Database,Essential,Genes,,DEG,,,contains,essential,genes,currently,available,functions,encoded,essential,genes,considered,foundation,life,therefore,likely,common,cells,Users,can,BLAST,query,sequences,DEG,homologous,genes,found,,possible,queried,genes,also,essential,Users,can,search,essential,genes,function,name,Users,can,also,browse,extract,records,DEG,Essential,gene,products,comprise,excellent,targets,antibacterial,drugs,Analysis,essential,genes,help,answer,question,basic,functions,necessary,support,cellular,life,DEG,freely,accessible,website,http://tubic.tju.edu.cn/deg/.DEG,5.0,,database,essential,genes,prokaryotes,eukaryotes,Essential,genes,indispensable,survival,organism,,functions,therefore,considered,foundation,life,Determination,minimal,gene,set,needed,sustain,life,form,,fundamental,question,biology,,plays,key,role,emerging,field,,synthetic,biology,Five,years,constructed,DEG,,database,essential,genes,,DEG,5.0,significant,advances,2004,version,number,essential,genes,number,organisms,genes,determined,number,prokaryotic,essential,genes,DEG,increased,10-fold,,mainly,owing,genome-wide,gene,essentiality,screens,performed,wide,range,bacteria,number,eukaryotic,essential,genes,increased,5-fold,,DEG,1.0,yeast,ones,,DEG,5.0,also,humans,,mice,,worms,,fruit,flies,,zebrafish,plant,Arabidopsis,thaliana,updates,represent,significant,advances,DEG,,also,represent,rapid,progress,essential-gene,field,DEG,freely,available,website,http://tubic.tju.edu.cn/deg,http://www.essentialgene.org.Gene,essentiality,analysis,based,DEG,10,,updated,database,essential,genes,database,essential,genes,,DEG,,available,http://www.essentialgene.org,,,constructed,2003,,timely,updated,harbor,essential-gene,records,bacteria,,archaea,,eukaryotes,DEG,10,,current,release,,includes,essential,protein-coding,genes,determined,genome-wide,gene,essentiality,screens,also,essential,noncoding,RNAs,,promoters,,regulatory,sequences,,replication,origins,Therefore,,DEG,10,includes,essential,genomic,elements,different,conditions,three,domains,life,,customizable,BLAST,tools,Based,analysis,DEG,10,,show,percentage,essential,genes,bacterial,genomes,exhibits,exponential,decay,increasing,genome,sizes,functions,,ATP,binding,,GO:0005524,,,GTP,binding,,GO:0005525,,,DNA-directed,RNA,polymerase,activity,,GO:0003899,,,likely,required,organisms,across,life,domains.<br>DEG,15,,update,Database,Essential,Genes,includes,built-in,analysis,tools,Essential,genes,refer,genes,required,organism,survive,specific,conditions,Studies,minimal-gene-set,bacteria,elucidated,fundamental,cellular,processes,sustain,life,past,five,years,seen,significant,progress,identifying,human,essential,genes,,primarily,due,successful,use,CRISPR/Cas9,various,types,human,cells,DEG,15,,new,release,Database,Essential,Genes,,www.essentialgene.org,,,provided,major,advancements,,compared,DEG,10,Specifically,,number,eukaryotic,essential,genes,increased,fourfold,,prokaryotic,ones,doubled,note,,human,essential-gene,number,increased,tenfold,Moreover,,developed,built-in,analysis,modules,users,can,perform,various,analyses,,essential-gene,distributions,bacterial,leading,lagging,strands,,sub-cellular,localization,distribution,,enrichment,analysis,gene,ontology,KEGG,pathways,,generation,Venn,diagrams,compare,contrast,gene,sets,experiments,Additionally,,database,offers,customizable,BLAST,tools,performing,species-,experiment-specific,BLAST,searches,Therefore,,DEG,comprehensively,harbors,updated,human-curated,essential-gene,records,among,prokaryotes,eukaryotes,built-in,tools,enhance,essential-gene,analysis.,DEG,10,,update,database,essential,genes,includes,protein-coding,genes,noncoding,genomic,elements,combination,high-density,transposon-mediated,mutagenesis,high-throughput,sequencing,led,significant,advancements,research,essential,genes,,resulting,dramatic,increase,number,identified,prokaryotic,essential,genes,diverse,conditions,revised,essential-gene,concept,includes,essential,genomic,elements,,rather,focusing,protein-coding,genes,DEG,10,,new,release,Database,Essential,Genes,,available,http://www.essentialgene.org,,,developed,accommodate,quantitative,qualitative,advancements,addition,increasing,number,bacterial,archaeal,essential,genes,determined,genome-wide,gene,essentiality,screens,,DEG,10,also,harbors,essential,noncoding,RNAs,,promoters,,regulatory,sequences,replication,origins,essential,genomic,elements,determined,vitro,,also,vivo,,diverse,conditions,including,survival,,pathogenesis,antibiotic,resistance,developed,customizable,BLAST,tools,allow,users,perform,species-,experiment-specific,BLAST,searches,single,gene,,list,genes,,annotated,unannotated,genomes,Therefore,,DEG,10,includes,essential,genomic,elements,different,conditions,three,domains,life,,customizable,BLAST,tools,DEG:,database,essential,genes,Essential,genes,genes,indispensable,support,cellular,life,genes,constitute,minimal,gene,set,required,living,cell,constructed,Database,Essential,Genes,,DEG,,,contains,essential,genes,currently,available,functions,encoded,essential,genes,considered,foundation,life,therefore,likely,common,cells,Users,can,BLAST,query,sequences,DEG,homologous,genes,found,,possible,queried,genes,also,essential,Users,can,search,essential,genes,function,name,Users,can,also,browse,extract,records,DEG,Essential,gene,products,comprise,excellent,targets,antibacterial,drugs,Analysis,essential,genes,help,answer,question,basic,functions,necessary,support,cellular,life,DEG,freely,accessible,website,http://tubic.tju.edu.cn/deg/.DEG,5.0,,database,essential,genes,prokaryotes,eukaryotes,Essential,genes,indispensable,survival,organism,,functions,therefore,considered,foundation,life,Determination,minimal,gene,set,needed,sustain,life,form,,fundamental,question,biology,,plays,key,role,emerging,field,,synthetic,biology,Five,years,constructed,DEG,,database,essential,genes,,DEG,5.0,significant,advances,2004,version,number,essential,genes,number,organisms,genes,determined,number,prokaryotic,essential,genes,DEG,increased,10-fold,,mainly,owing,genome-wide,gene,essentiality,screens,performed,wide,range,bacteria,number,eukaryotic,essential,genes,increased,5-fold,,DEG,1.0,yeast,ones,,DEG,5.0,also,humans,,mice,,worms,,fruit,flies,,zebrafish,plant,Arabidopsis,thaliana,updates,represent,significant,advances,DEG,,also,represent,rapid,progress,essential-gene,field,DEG,freely,available,website,http://tubic.tju.edu.cn/deg,http://www.essentialgene.org.Gene,essentiality,analysis,based,DEG,10,,updated,database,essential,genes,database,essential,genes,,DEG,,available,http://www.essentialgene.org,,,constructed,2003,,timely,updated,harbor,essential-gene,records,bacteria,,archaea,,eukaryotes,DEG,10,,current,release,,includes,essential,protein-coding,genes,determined,genome-wide,gene,essentiality,screens,also,essential,noncoding,RNAs,,promoters,,regulatory,sequences,,replication,origins,Therefore,,DEG,10,includes,essential,genomic,elements,different,conditions,three,domains,life,,customizable,BLAST,tools,Based,analysis,DEG,10,,show,percentage,essential,genes,bacterial,genomes,exhibits,exponential,decay,increasing,genome,sizes,functions,,ATP,binding,,GO:0005524,,,GTP,binding,,GO:0005525,,,DNA-directed,RNA,polymerase,activity,,GO:0003899,,,likely,required,organisms,across,life,domains.<br>DEG,15,,update,Database,Essential,Genes,includes,built-in,analysis,tools,Essential,genes,refer,genes,required,organism,survive,specific,conditions,Studies,minimal-gene-set,bacteria,elucidated,fundamental,cellular,processes,sustain,life,past,five,years,seen,significant,progress,identifying,human,essential,genes,,primarily,due,successful,use,CRISPR/Cas9,various,types,human,cells,DEG,15,,new,release,Database,Essential,Genes,,www.essentialgene.org,,,provided,major,advancements,,compared,DEG,10,Specifically,,number,eukaryotic,essential,genes,increased,fourfold,,prokaryotic,ones,doubled,note,,human,essential-gene,number,increased,tenfold,Moreover,,developed,built-in,analysis,modules,users,can,perform,various,analyses,,essential-gene,distributions,bacterial,leading,lagging,strands,,sub-cellular,localization,distribution,,enrichment,analysis,gene,ontology,KEGG,pathways,,generation,Venn,diagrams,compare,contrast,gene,sets,experiments,Additionally,,database,offers,customizable,BLAST,tools,performing,species-,experiment-specific,BLAST,searches,Therefore,,DEG,comprehensively,harbors,updated,human-curated,essential-gene,records,among,prokaryotes,eukaryotes,built-in,tools,enhance,essential-gene,analysis.