CGKB,CGKB,CGKB,CGKB,CGKB,CGKB,CGKB,CGKB,CGKB,CGKB,CGKB:,annotation,knowledge,base,cowpea,,Vigna,unguiculata,L.,,methylation,filtered,genomic,genespace,sequences,BACKGROUND:,Cowpea,[Vigna,unguiculata,,L.,,Walp.],one,important,food,forage,legumes,semi-arid,tropics,ability,tolerate,drought,grow,poor,soils,cultivated,mostly,poor,farmers,developing,countries,,80%,production,taking,place,dry,savannah,tropical,West,Central,Africa,Cowpea,largely,underexploited,crop,relatively,little,genomic,information,available,use,applied,plant,breeding,goal,Cowpea,Genomics,Initiative,,CGI,,,funded,Kirkhouse,Trust,,UK-based,charitable,organization,,leverage,modern,molecular,genetic,tools,gene,discovery,cowpea,improvement,One,aspect,initiative,sequencing,gene-rich,region,cowpea,genome,,termed,genespace,,recovered,using,methylation,filtration,technology,providing,annotation,analysis,sequence,data.<br>DESCRIPTION:,CGKB,,Cowpea,Genespace/Genomics,Knowledge,Base,,annotation,knowledge,base,developed,CGI,database,based,information,derived,298,848,cowpea,genespace,sequences,,GSS,,isolated,methylation,filtering,genomic,DNA,CGKB,consists,three,knowledge,bases:,GSS,annotation,comparative,genomics,knowledge,base,,GSS,enzyme,metabolic,pathway,knowledge,base,,GSS,simple,sequence,repeats,,SSRs,,knowledge,base,molecular,marker,discovery,homology-based,approach,applied,annotations,GSS,,mainly,using,BLASTX,four,public,FASTA,formatted,protein,databases,,NCBI,GenBank,Proteins,,UniProtKB-Swiss-Prot,,UniprotKB-PIR,,Protein,Information,Resource,,,UniProtKB-TrEMBL,,Comparative,genome,analysis,done,BLASTX,searches,cowpea,GSS,four,plant,proteomes,Arabidopsis,thaliana,,Oryza,sativa,,Medicago,truncatula,,Populus,trichocarpa,possible,exons,introns,cowpea,GSS,predicted,using,HMM-based,Genscan,gene,predication,program,potential,domains,annotated,GSS,analyzed,using,HMMER,package,Pfam,database,annotated,GSS,also,assigned,Gene,Ontology,annotation,terms,integrated,228,curated,plant,metabolic,pathways,Arabidopsis,Information,Resource,,TAIR,,knowledge,base,UniProtKB-Swiss-Prot,ENZYME,database,used,assign,putative,enzymatic,function,GSS,GSS,also,analyzed,Tandem,Repeat,Finder,,TRF,,program,order,identify,potential,SSRs,molecular,marker,discovery,raw,sequence,data,,processed,annotation,,SSR,results,stored,relational,tables,designed,key-value,pair,fashion,using,PostgreSQL,relational,database,management,system,biological,knowledge,derived,sequence,data,processed,results,represented,views,materialized,views,relational,database,management,system,materialized,views,indexed,quick,data,access,retrieval,Data,processing,analysis,pipelines,implemented,using,Perl,programming,language,web,interface,implemented,JavaScript,Perl,CGI,running,Apache,web,server,CPU,intensive,data,processing,analysis,pipelines,run,computer,cluster,30,dual-processor,Apple,XServes,job,management,system,called,Vela,created,robust,way,submit,large,numbers,jobs,Portable,Batch,System,,PBS,.<br>CONCLUSION:,CGKB,integrated,annotated,resource,cowpea,GSS,features,homology-based,HMM-based,annotations,,enzyme,pathway,annotations,,GO,term,annotation,,toolkits,,large,number,facilities,perform,complex,queries,cowpea,GSS,,chloroplast,sequences,,mitochondrial,sequences,,retroelements,,SSR,sequences,available,FASTA,formatted,files,downloadable,CGKB,database,web,interface,publicly,accessible,http://cowpeagenomics.med.virginia.edu/CGKB/.<br>,CGKB:,annotation,knowledge,base,cowpea,,Vigna,unguiculata,L.,,methylation,filtered,genomic,genespace,sequences,BACKGROUND:,Cowpea,[Vigna,unguiculata,,L.,,Walp.],one,important,food,forage,legumes,semi-arid,tropics,ability,tolerate,drought,grow,poor,soils,cultivated,mostly,poor,farmers,developing,countries,,80%,production,taking,place,dry,savannah,tropical,West,Central,Africa,Cowpea,largely,underexploited,crop,relatively,little,genomic,information,available,use,applied,plant,breeding,goal,Cowpea,Genomics,Initiative,,CGI,,,funded,Kirkhouse,Trust,,UK-based,charitable,organization,,leverage,modern,molecular,genetic,tools,gene,discovery,cowpea,improvement,One,aspect,initiative,sequencing,gene-rich,region,cowpea,genome,,termed,genespace,,recovered,using,methylation,filtration,technology,providing,annotation,analysis,sequence,data.<br>DESCRIPTION:,CGKB,,Cowpea,Genespace/Genomics,Knowledge,Base,,annotation,knowledge,base,developed,CGI,database,based,information,derived,298,848,cowpea,genespace,sequences,,GSS,,isolated,methylation,filtering,genomic,DNA,CGKB,consists,three,knowledge,bases:,GSS,annotation,comparative,genomics,knowledge,base,,GSS,enzyme,metabolic,pathway,knowledge,base,,GSS,simple,sequence,repeats,,SSRs,,knowledge,base,molecular,marker,discovery,homology-based,approach,applied,annotations,GSS,,mainly,using,BLASTX,four,public,FASTA,formatted,protein,databases,,NCBI,GenBank,Proteins,,UniProtKB-Swiss-Prot,,UniprotKB-PIR,,Protein,Information,Resource,,,UniProtKB-TrEMBL,,Comparative,genome,analysis,done,BLASTX,searches,cowpea,GSS,four,plant,proteomes,Arabidopsis,thaliana,,Oryza,sativa,,Medicago,truncatula,,Populus,trichocarpa,possible,exons,introns,cowpea,GSS,predicted,using,HMM-based,Genscan,gene,predication,program,potential,domains,annotated,GSS,analyzed,using,HMMER,package,Pfam,database,annotated,GSS,also,assigned,Gene,Ontology,annotation,terms,integrated,228,curated,plant,metabolic,pathways,Arabidopsis,Information,Resource,,TAIR,,knowledge,base,UniProtKB-Swiss-Prot,ENZYME,database,used,assign,putative,enzymatic,function,GSS,GSS,also,analyzed,Tandem,Repeat,Finder,,TRF,,program,order,identify,potential,SSRs,molecular,marker,discovery,raw,sequence,data,,processed,annotation,,SSR,results,stored,relational,tables,designed,key-value,pair,fashion,using,PostgreSQL,relational,database,management,system,biological,knowledge,derived,sequence,data,processed,results,represented,views,materialized,views,relational,database,management,system,materialized,views,indexed,quick,data,access,retrieval,Data,processing,analysis,pipelines,implemented,using,Perl,programming,language,web,interface,implemented,JavaScript,Perl,CGI,running,Apache,web,server,CPU,intensive,data,processing,analysis,pipelines,run,computer,cluster,30,dual-processor,Apple,XServes,job,management,system,called,Vela,created,robust,way,submit,large,numbers,jobs,Portable,Batch,System,,PBS,.<br>CONCLUSION:,CGKB,integrated,annotated,resource,cowpea,GSS,features,homology-based,HMM-based,annotations,,enzyme,pathway,annotations,,GO,term,annotation,,toolkits,,large,number,facilities,perform,complex,queries,cowpea,GSS,,chloroplast,sequences,,mitochondrial,sequences,,retroelements,,SSR,sequences,available,FASTA,formatted,files,downloadable,CGKB,database,web,interface,publicly,accessible,http://cowpeagenomics.med.virginia.edu/CGKB/.<br>