zTrap,zTrap,zTrap,zTrap,zTrap,zTrap,zTrap,zTrap,zTrap,zTrap,zTrap:,zebrafish,gene,trap,enhancer,trap,database,BACKGROUND:,developed,genetic,methods,zebrafish,using,Tol2,transposable,element;,namely,,transgenesis,,gene,trapping,,enhancer,trapping,Gal4FF-UAS,system,Gene,trap,constructs,contain,splice,acceptor,GFP,Gal4FF,,modified,version,yeast,Gal4,transcription,activator,,gene,,enhancer,trap,constructs,contain,zebrafish,hsp70l,promoter,GFP,Gal4FF,gene,performing,genetic,screens,using,constructs,,generated,transgenic,zebrafish,express,GFP,Gal4FF,specific,cells,,tissues,organs,Gal4FF,expression,visualized,creating,double,transgenic,fish,carrying,Gal4FF,transgene,GFP,reporter,gene,placed,downstream,Gal4-recognition,sequence,,UAS,,,Gal4FF-expressing,cells,can,manipulated,mating,UAS,effector,fish,instance,,fish,expressing,Gal4FF,specific,neurons,crossed,UAS:TeTxLC,fish,carrying,tetanus,neurotoxin,gene,downstream,UAS,,neuronal,activities,inhibited,double,transgenic,fish,Thus,,transgenic,fish,useful,study,developmental,biology,neurobiology.<br>DESCRIPTION:,increase,usefulness,transgenic,fish,resource,,developed,web-based,database,named,zTrap,http://kawakami.lab.nig.ac.jp/ztrap/,zTrap,database,contains,images,GFP,Gal4FF,expression,patterns,,genomic,DNA,sequences,surrounding,integration,sites,gene,trap,enhancer,trap,constructs,integration,sites,mapped,onto,Ensembl,zebrafish,genome,in-house,Blat,analysis,can,viewed,zTrap,Ensembl,genome,browsers,Furthermore,,zTrap,equipped,functionality,search,data,expression,patterns,genomic,loci,interest,zTrap,contains,information,transgenic,fish,including,UAS,reporter,effector,fish.<br>CONCLUSION:,zTrap,useful,resource,find,gene,trap,enhancer,trap,fish,lines,express,GFP,Gal4FF,desired,patterns,,find,insertions,gene,trap,enhancer,trap,constructs,located,within,near,genes,interest,transgenic,fish,can,utilized,observe,specific,cell,types,embryogenesis,,manipulate,functions,,discover,novel,genes,cis-regulatory,elements,Therefore,,zTrap,facilitate,studies,genomics,,developmental,biology,neurobiology,utilizing,transgenic,zebrafish,resource.<br>,zTrap:,zebrafish,gene,trap,enhancer,trap,database,BACKGROUND:,developed,genetic,methods,zebrafish,using,Tol2,transposable,element;,namely,,transgenesis,,gene,trapping,,enhancer,trapping,Gal4FF-UAS,system,Gene,trap,constructs,contain,splice,acceptor,GFP,Gal4FF,,modified,version,yeast,Gal4,transcription,activator,,gene,,enhancer,trap,constructs,contain,zebrafish,hsp70l,promoter,GFP,Gal4FF,gene,performing,genetic,screens,using,constructs,,generated,transgenic,zebrafish,express,GFP,Gal4FF,specific,cells,,tissues,organs,Gal4FF,expression,visualized,creating,double,transgenic,fish,carrying,Gal4FF,transgene,GFP,reporter,gene,placed,downstream,Gal4-recognition,sequence,,UAS,,,Gal4FF-expressing,cells,can,manipulated,mating,UAS,effector,fish,instance,,fish,expressing,Gal4FF,specific,neurons,crossed,UAS:TeTxLC,fish,carrying,tetanus,neurotoxin,gene,downstream,UAS,,neuronal,activities,inhibited,double,transgenic,fish,Thus,,transgenic,fish,useful,study,developmental,biology,neurobiology.<br>DESCRIPTION:,increase,usefulness,transgenic,fish,resource,,developed,web-based,database,named,zTrap,http://kawakami.lab.nig.ac.jp/ztrap/,zTrap,database,contains,images,GFP,Gal4FF,expression,patterns,,genomic,DNA,sequences,surrounding,integration,sites,gene,trap,enhancer,trap,constructs,integration,sites,mapped,onto,Ensembl,zebrafish,genome,in-house,Blat,analysis,can,viewed,zTrap,Ensembl,genome,browsers,Furthermore,,zTrap,equipped,functionality,search,data,expression,patterns,genomic,loci,interest,zTrap,contains,information,transgenic,fish,including,UAS,reporter,effector,fish.<br>CONCLUSION:,zTrap,useful,resource,find,gene,trap,enhancer,trap,fish,lines,express,GFP,Gal4FF,desired,patterns,,find,insertions,gene,trap,enhancer,trap,constructs,located,within,near,genes,interest,transgenic,fish,can,utilized,observe,specific,cell,types,embryogenesis,,manipulate,functions,,discover,novel,genes,cis-regulatory,elements,Therefore,,zTrap,facilitate,studies,genomics,,developmental,biology,neurobiology,utilizing,transgenic,zebrafish,resource.<br>