Lotus japonicus EST Index,Lotus japonicus EST Index,Lotus japonicus EST Index,Lotus japonicus EST Index,Lotus japonicus EST Index,Lotus japonicus EST Index,Lotus japonicus EST Index,Lotus japonicus EST Index,Lotus japonicus EST Index,Lotus japonicus EST Index,Characteristics,Lotus,japonicus,gene,repertoire,deduced,large-scale,expressed,sequence,tag,,EST,,analysis,perform,comprehensive,analysis,genes,expressed,model,legume,,Lotus,japonicus,,total,74472,3'-end,expressed,sequence,tags,,EST,,generated,cDNA,libraries,produced,six,different,organs,Clustering,sequences,performed,identity,criterion,95%,50,bases,,total,20457,non-redundant,sequences,,8503,contigs,11954,singletons,generated,EST,sequence,coverage,analyzed,using,annotated,L,japonicus,genomic,sequence,1093,1889,predicted,protein-encoding,genes,,57.9%,,hit,EST,sequence,s,,Gene,content,compared,several,plant,species,Among,8503,contigs,,471,identified,sequences,conserved,leguminous,species,included,several,disease,resistance-related,genes,suggested,legumes,,genes,may,evolved,specifically,resist,pathogen,attack,rate,gene,sequence,divergence,assessed,comparing,similarity,level,functional,category,based,Gene,Ontology,,GO,,annotation,Arabidopsis,genes,revealed,genes,encoding,ribosomal,proteins,,well,related,translation,,photosynthesis,,cellular,structure,abundantly,represented,highly,conserved,class,,genes,encoding,transcription,factors,receptor,protein,kinases,abundantly,represented,less,conserved,class,make,sequence,information,cDNA,clones,available,research,community,,Web,database,useful,services,created,http://www.kazusa.or.jp/en/plant/lotus/EST/.<br>Generation,7137,non-redundant,expressed,sequence,tags,legume,,Lotus,japonicus,comprehensive,analysis,genes,expressed,model,legume,,Lotus,japonicus,,total,22,983,5',end,expressed,sequence,tags,,ESTs,,accumulated,normalized,size-selected,cDNA,libraries,constructed,young,,2,weeks,old,,plants,EST,sequences,clustered,7137,non-redundant,groups,Similarity,search,public,non-redundant,protein,database,indicated,3302,groups,showed,similarity,genes,known,function,,1143,groups,hypothetical,genes,,2692,novel,sequences,Homologues,5,nodule-specific,genes,reported,legume,species,contained,collected,ESTs,,suggesting,EST,source,generated,study,will,become,useful,tool,identification,genes,related,legume-specific,biological,processes,sequence,data,individual,ESTs,available,web,site:,http://www.kazusa.or.jp/en/plant/lotus/EST/.<br>Analysis,expressed,sequence,tags,flower,buds,Lotus,japonicus,order,study,gene,expression,reproductive,organ,,constructed,cDNA,library,mature,flower,buds,Lotus,japonicus,,characterized,expressed,sequence,tags,,ESTs,,842,clones,randomly,selected,EST,sequences,clustered,718,non-redundant,groups,BLAST,FASTA,search,analyses,protein,DNA,databases,,58.5%,EST,groups,showed,significant,sequence,similarities,known,genes,Several,genes,encoding,EST,clones,identified,pollen-specific,genes,,pectin,methylesterase,,ascorbate,oxidase,,polygalacturonase,,homologous,genes,involved,pollen-pistil,interaction,Comparison,EST,sequences,derived,whole,plant,L,japonicus,,revealed,64.8%,EST,sequences,flower,buds,found,EST,sequences,whole,plant,Taken,together,,EST,data,flower,buds,generated,study,useful,dissecting,gene,expression,floral,organ,L,japonicus.<br>,Characteristics,Lotus,japonicus,gene,repertoire,deduced,large-scale,expressed,sequence,tag,,EST,,analysis,perform,comprehensive,analysis,genes,expressed,model,legume,,Lotus,japonicus,,total,74472,3'-end,expressed,sequence,tags,,EST,,generated,cDNA,libraries,produced,six,different,organs,Clustering,sequences,performed,identity,criterion,95%,50,bases,,total,20457,non-redundant,sequences,,8503,contigs,11954,singletons,generated,EST,sequence,coverage,analyzed,using,annotated,L,japonicus,genomic,sequence,1093,1889,predicted,protein-encoding,genes,,57.9%,,hit,EST,sequence,s,,Gene,content,compared,several,plant,species,Among,8503,contigs,,471,identified,sequences,conserved,leguminous,species,included,several,disease,resistance-related,genes,suggested,legumes,,genes,may,evolved,specifically,resist,pathogen,attack,rate,gene,sequence,divergence,assessed,comparing,similarity,level,functional,category,based,Gene,Ontology,,GO,,annotation,Arabidopsis,genes,revealed,genes,encoding,ribosomal,proteins,,well,related,translation,,photosynthesis,,cellular,structure,abundantly,represented,highly,conserved,class,,genes,encoding,transcription,factors,receptor,protein,kinases,abundantly,represented,less,conserved,class,make,sequence,information,cDNA,clones,available,research,community,,Web,database,useful,services,created,http://www.kazusa.or.jp/en/plant/lotus/EST/.<br>Generation,7137,non-redundant,expressed,sequence,tags,legume,,Lotus,japonicus,comprehensive,analysis,genes,expressed,model,legume,,Lotus,japonicus,,total,22,983,5',end,expressed,sequence,tags,,ESTs,,accumulated,normalized,size-selected,cDNA,libraries,constructed,young,,2,weeks,old,,plants,EST,sequences,clustered,7137,non-redundant,groups,Similarity,search,public,non-redundant,protein,database,indicated,3302,groups,showed,similarity,genes,known,function,,1143,groups,hypothetical,genes,,2692,novel,sequences,Homologues,5,nodule-specific,genes,reported,legume,species,contained,collected,ESTs,,suggesting,EST,source,generated,study,will,become,useful,tool,identification,genes,related,legume-specific,biological,processes,sequence,data,individual,ESTs,available,web,site:,http://www.kazusa.or.jp/en/plant/lotus/EST/.<br>Analysis,expressed,sequence,tags,flower,buds,Lotus,japonicus,order,study,gene,expression,reproductive,organ,,constructed,cDNA,library,mature,flower,buds,Lotus,japonicus,,characterized,expressed,sequence,tags,,ESTs,,842,clones,randomly,selected,EST,sequences,clustered,718,non-redundant,groups,BLAST,FASTA,search,analyses,protein,DNA,databases,,58.5%,EST,groups,showed,significant,sequence,similarities,known,genes,Several,genes,encoding,EST,clones,identified,pollen-specific,genes,,pectin,methylesterase,,ascorbate,oxidase,,polygalacturonase,,homologous,genes,involved,pollen-pistil,interaction,Comparison,EST,sequences,derived,whole,plant,L,japonicus,,revealed,64.8%,EST,sequences,flower,buds,found,EST,sequences,whole,plant,Taken,together,,EST,data,flower,buds,generated,study,useful,dissecting,gene,expression,floral,organ,L,japonicus.<br>