DeepHF,DeepHF,DeepHF,DeepHF,DeepHF,DeepHF,DeepHF,DeepHF,DeepHF,DeepHF,Optimized,CRISPR,guide,RNA,design,two,high-fidelity,Cas9,variants,deep,learning,Highly,specific,Cas9,nucleases,derived,SpCas9,valuable,tools,genome,editing,,wide,applications,hampered,lack,knowledge,governing,guide,RNA,,gRNA,,activity,,perform,genome-scale,screen,measure,gRNA,activity,two,highly,specific,SpCas9,variants,,eSpCas9,1.1,,SpCas9-HF1,,wild-type,SpCas9,,WT-SpCas9,,human,cells,,obtain,indel,rates,50,000,gRNAs,nuclease,,covering,~20,000,genes,evaluate,contribution,1,031,features,gRNA,activity,develope,models,activity,prediction,data,reveals,combination,RNN,important,biological,features,outperforms,models,activity,prediction,demonstrate,model,outperforms,popular,gRNA,design,tools,Finally,,develop,online,design,tool,DeepHF,three,Cas9,nucleases,database,,well,designer,tool,,freely,accessible,via,web,server,,http://www.DeepHF.com/,.<br>,Optimized,CRISPR,guide,RNA,design,two,high-fidelity,Cas9,variants,deep,learning,Highly,specific,Cas9,nucleases,derived,SpCas9,valuable,tools,genome,editing,,wide,applications,hampered,lack,knowledge,governing,guide,RNA,,gRNA,,activity,,perform,genome-scale,screen,measure,gRNA,activity,two,highly,specific,SpCas9,variants,,eSpCas9,1.1,,SpCas9-HF1,,wild-type,SpCas9,,WT-SpCas9,,human,cells,,obtain,indel,rates,50,000,gRNAs,nuclease,,covering,~20,000,genes,evaluate,contribution,1,031,features,gRNA,activity,develope,models,activity,prediction,data,reveals,combination,RNN,important,biological,features,outperforms,models,activity,prediction,demonstrate,model,outperforms,popular,gRNA,design,tools,Finally,,develop,online,design,tool,DeepHF,three,Cas9,nucleases,database,,well,designer,tool,,freely,accessible,via,web,server,,http://www.DeepHF.com/,.<br>