参考基因组: GCF_017639785.1_BCM_Maur_2.0
基因组注释: GCF_017639785.1_BCM_Maur_2.0
数据处理:
- Fastp v0.20.0; fastp -q 20 -u 40 -l 50 -g -x -r -W 4 -M 20
- RseQC v2.6.4; python infer_experiment.py -r $index -I $Projectid/$SRXid/${SRXid}_Alignment-unsorted.bam > $Projectid/$SRXid/${SRXid}_RseQC.txt
- STAR v2.7.1a, RSEM v1.3.1; rsem-calculate-expression --keep-intermediate-files --temporary-folder $Projectid/$SRXid/${SRXid}_STAR_rsem --paired-end --forward-prob=$value -p $thread --time --star --star-path /software/biosoft/software/STAR-2.7/STAR/bin/Linux_x86_64/ --append-names --output-genome-bam --sort-bam-by-coordinate --star-gzipped-read-file $Projectid/$SRXid/${SRXid}_clean_1.fastq.gz $Projectid/$SRXid/${SRXid}_clean_2.fastq.gz $index $Projectid/$SRXid/${SRXid}_star_RSEM