Gene Expression Nebulas
A data portal of transcriptomic profiles analyzed by a unified pipeline across multiple species

Gene Expression Nebulas

A data portal of transcriptome profiles across multiple species

PRJCA000248: Human Blastocyst Methylome

Source: NGDC / CRA000114
Submission Date: Jun 23 2016
Release Date: Oct 30 2018
Update Date: Oct 30 2018

Summary: We performed whole genome bisulfite sequencing (WGBS) for 57 blastocysts and 29 trophectoderm samples with different morphological grades during assisted reproductive technology(ART) practices.

GEN Datasets:
GEND000184
Strategy:
Species:
Tissue:
Healthy Condition:
Protocol
Growth Protocol: -
Treatment Protocol: -
Extract Protocol: -
Library Construction Protocol: RNA-Seq libraries were constructed with the NEBNext Ultra RNA Library Prep Kit for Illimina according to the manufactor^s instruction; mRNA-seq data for human blastocyst embryos, including AA and CC level.
Sequencing
Molecule Type: poly(A)+ RNA
Library Source:
Library Layout: PAIRED
Library Strand: -
Platform: ILLUMINA
Instrument Model: Illumina HiSeq 2500
Strand-Specific: Unspecific
Samples
Basic Information:
Sample Characteristic:
Biological Condition:
Experimental Variables:
Protocol:
Sequencing:
Assessing Quality:
Analysis:
Data Resource GEN Sample ID GEN Dataset ID Project ID BioProject ID Sample ID Sample Name BioSample ID Sample Accession Experiment Accession Release Date Submission Date Update Date Species Race Ethnicity Age Age Unit Gender Source Name Tissue Cell Type Cell Subtype Cell Line Disease Disease State Development Stage Mutation Phenotype Case Detail Control Detail Growth Protocol Treatment Protocol Extract Protocol Library Construction Protocol Molecule Type Library Layout Strand-Specific Library Strand Spike-In Strategy Platform Instrument Model Cell Number Reads Number Gbases AvgSpotLen1 AvgSpotLen2 Uniq Mapping Rate Multiple Mapping Rate Coverage Rate
Publications
Genome wide abnormal DNA methylome of human blastocyst in assisted reproductive technology.
Journal of genetics and genomics = Yi chuan xue bao . 2017-09-06 [PMID: 29037989]