Gene Expression Nebulas概要: Exploring regulatory mechanism of rice tiller angle through dynamic transcriptome of shoot gravitropism
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| 生长方案: | For RNA-seq and time-lapse photography, germinated ZH11 seeds were grown on 0.4% (w/v) agar plates at 28°C for 3 d under a 16-h-light/8-h-dark photoperiod, and then seedlings with uniform growth were transferred into soil and grown under controlled greenhouse conditions (16 h light and 8 h dark at 28°C, 150-200 μmol photons m-2 s-1) for 11 d prior to the gravistimulation treatments. |
| 处理方案: | Shoot bases (1.5 cm in length) were harvested at 12 time points (0, 0.25, 0.5, 1, 1.5, 2, 3, 4, 5, 6, 7, and 8 h) after the start of gravistimulation and were frozen immediately in liquid nitrogen. |
| 提取方案: | RNA was extracted using a TRIzol kit according to the user manual (Invitrogen). |
| 建库方案: | Illumina Part #15026495 Rev.D |
| 分子类型: | poly(A)+ RNA |
| 库的片段类型: | Single |
| 库的链类型: | - |
| 测序平台: | ILLUMINA |
| 测序仪型号: | Illumina HiSeq 2000 |
| 链特异性: | Unspecific |
| 数据来源 | GEN样本编号 | GEN数据集编号 | 系列编号 | 项目编号 | 样本编号 | 样本名称 | 生物样本编号 | 样本访问号 | 实验访问号 | 释放时间 | 提交时间 | 最后更新时间 | 物种 | 种族 | 族裔 | 年龄 | 年龄单位 | 性别 | 来源名称 | 组织 | 细胞类型 | 细胞亚型 | 细胞系 | 疾病 | 疾病状态 | 发育阶段 | 突变/变异 | 表型 | Condition Detail | 生长方案 | 处理方案 | 提取方案 | 建库方案 | 分子类型 | 库的片段类型 | 链特异性 | 库的链类型 | 加标(Spike-In) | 测序方法 | 测序平台 | 测序仪型号 | 细胞数 | 测序片段数 | 碱基数 | 平均测序片段长度_1 | 平均测序片段长度_2 | 唯一比对率 | 多重比对率 | 覆盖度 |
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