Gene Expression
Nebulas
Gene Expression NebulasSummary: Chromatin accessibility landscape by characterizing DNase I hypersensitive sites (DHSs) in human embryos
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| Growth Protocol: | - |
| Treatment Protocol: | - |
| Extract Protocol: | The embryos were lysed directly and used for cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, 634888). |
| Library Construction Protocol: | The cDNA samples were fragmented to 200-400bp by Covaris sonicator (Covaris). Sequencing libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S). |
| Molecule Type: | poly(A)+ RNA |
| Library Source: | |
| Library Layout: | PAIRED |
| Library Strand: | - |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina HiSeq X Ten |
| Strand-Specific: | Unspecific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Condition Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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