Gene Expression
Nebulas
Gene Expression NebulasSummary: 1, detect the gene expresion profile of Zika virus infected human colonal epithelial cells (HT-29); 2, detect the azithromycin upregulated genes in HT-29 cells after Zika virus infection
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| Growth Protocol: | - |
| Treatment Protocol: | - |
| Extract Protocol: | - |
| Library Construction Protocol: | RNA was used to remove ribosomal RNA with the Ribo-Zero Magnetic Gold Kit followed by a cleanup with Agencourt RNAClean XP beads and elution by RNA Fragmentation and Priming mix from the NEBNext Ultra Directional RNA Library Prep Kit for Illumina. |
| Molecule Type: | rRNA- RNA |
| Library Source: | |
| Library Layout: | PAIRED |
| Library Strand: | Forward |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina HiSeq X Ten |
| Strand-Specific: | Specific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Condition Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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