Gene Expression Nebulas
A data portal of transcriptomic profiles analyzed by a unified pipeline across multiple species

Gene Expression Nebulas

A data portal of transcriptome profiles across multiple species

PRJCA000776: Gene expression profile of Zika virus infected HT-29 cells

Source: NGDC / CRA000783
Submission Date: Mar 02 2018
Release Date: Aug 7 2018
Update Date: Aug 7 2018

Summary: 1, detect the gene expresion profile of Zika virus infected human colonal epithelial cells (HT-29); 2, detect the azithromycin upregulated genes in HT-29 cells after Zika virus infection

GEN Datasets:
GEND000186
Strategy:
Species:
Healthy Condition:
Cell Type:
Cell Line:
Protocol
Growth Protocol: -
Treatment Protocol: -
Extract Protocol: -
Library Construction Protocol: RNA was used to remove ribosomal RNA with the Ribo-Zero Magnetic Gold Kit followed by a cleanup with Agencourt RNAClean XP beads and elution by RNA Fragmentation and Priming mix from the NEBNext Ultra Directional RNA Library Prep Kit for Illumina.
Sequencing
Molecule Type: rRNA- RNA
Library Source:
Library Layout: PAIRED
Library Strand: Forward
Platform: ILLUMINA
Instrument Model: Illumina HiSeq X Ten
Strand-Specific: Specific
Samples
Basic Information:
Sample Characteristic:
Biological Condition:
Experimental Variables:
Protocol:
Sequencing:
Assessing Quality:
Analysis:
Data Resource GEN Sample ID GEN Dataset ID Project ID BioProject ID Sample ID Sample Name BioSample ID Sample Accession Experiment Accession Release Date Submission Date Update Date Species Race Ethnicity Age Age Unit Gender Source Name Tissue Cell Type Cell Subtype Cell Line Disease Disease State Development Stage Mutation Phenotype Case Detail Control Detail Growth Protocol Treatment Protocol Extract Protocol Library Construction Protocol Molecule Type Library Layout Strand-Specific Library Strand Spike-In Strategy Platform Instrument Model Cell Number Reads Number Gbases AvgSpotLen1 AvgSpotLen2 Uniq Mapping Rate Multiple Mapping Rate Coverage Rate
Publications
Zika virus shedding in the stool and infection through the anorectal mucosa in mice.
Emerging microbes & infections . 2018-10-17 [PMID: 30333476]
Azithromycin Protects against Zika virus Infection by Upregulating virus-induced Type I and III Interferon Responses.
Antimicrobial agents and chemotherapy . 2019-09-16 [PMID: 31527024]