| 生长方案: |
Arabidopsis plants used for protoplast preparation, insect feeding, and the pathogen infection assays were grown in soil (vermiculite:nutrition soil ratio of 1:1) at 22°C with a short-day (10-h light/14-h dark) photoperiod for 4 weeks. Other Arabidopsis plants were grown at 22°C under a long-day (16 h light/8 h dark) photoperiod (light intensity of 120 μmol photons m-2 s-1). |
| 处理方案: |
The 10-d-old wild-type and luh-4 seedlings were treated with 100 μM MeJA for 1, 6, or 24 h. The untreated wild-type and luh-4 seedlings were used as controls. |
| 提取方案: |
Total RNA of each sample was extracted using an RNeasy plant Mini Kit (Qiagen) and treated with DNase I. The quality of the total RNA was assessed using a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer. |
| 建库方案: |
For each sample, 3 μg of total RNA was used to construct the Illumina sequencing libraries according to the manufacturer’s instructions. |