Growth Protocol:	Arabidopsis plants used for protoplast preparation, insect feeding, and the pathogen infection assays were grown in soil (vermiculite:nutrition soil ratio of 1:1) at 22°C with a short-day (10-h light/14-h dark) photoperiod for 4 weeks. Other Arabidopsis plants were grown at 22°C under a long-day (16 h light/8 h dark) photoperiod (light intensity of 120 μmol photons m-2 s-1).
Treatment Protocol:	The 10-d-old wild-type and luh-4 seedlings were treated with 100 μM MeJA for 1, 6, or 24 h. The untreated wild-type and luh-4 seedlings were used as controls.
Extract Protocol:	Total RNA of each sample was extracted using an RNeasy plant Mini Kit (Qiagen) and treated with DNase I. The quality of the total RNA was assessed using a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer.
Library Construction Protocol:	For each sample, 3 μg of total RNA was used to construct the Illumina sequencing libraries according to the manufacturer’s instructions.

Molecule Type:	-
Library Source:
Library Layout:	PAIRED
Library Strand:	-
Platform:	ILLUMINA
Instrument Model:	Illumina HiSeq 2500
Strand-Specific:	Unspecific

Data Resource	GEN Sample ID	GEN Dataset ID	Project ID	BioProject ID	Sample ID	Sample Name	BioSample ID	Sample Accession	Experiment Accession	Release Date	Submission Date	Update Date	Species	Race	Ethnicity	Age	Age Unit	Gender	Source Name	Tissue	Cell Type	Cell Subtype	Cell Line	Disease	Disease State	Development Stage	Mutation	Phenotype	Case Detail	Control Detail	Growth Protocol	Treatment Protocol	Extract Protocol	Library Construction Protocol	Molecule Type	Library Layout	Strand-Specific	Library Strand	Spike-In	Strategy	Platform	Instrument Model	Cell Number	Reads Number	Gbases	AvgSpotLen1	AvgSpotLen2	Uniq Mapping Rate	Multiple Mapping Rate	Coverage Rate