Gene Expression Nebulas
基于标准化流程分析的转录图谱综合数据库

Gene Expression Nebulas

多物种转录图谱整合数据库

PRJCA002998: The RNA-seq data of fibre development in two upland cotton cultivars (Nongdamian13 and Nongda601)

来源: NGDC / CRA002927
提交时间: Jul 02 2020
释放时间: Jul 03 2020
最后更新时间: -

概要: To detect the changes of the genes expression during the fiber development stage

项目整体设计: Growing bolls from the two upland cotton cultivars nongdamian13 (high quality) and Nongda601 (normal quality) were collected at the initiation stage (0 DPA, day post anthesis), cell-elongation stage (5, 10, 15 DPA), and secondary-wall synthesis stage (20, 25, and 30 DPA). The qualified RNA was extracted from these samples and applied for the RNA-seq.

GEN 数据集:
GEND000318
测序方法:
物种:
组织:
发育阶段:
方案
生长方案: -
处理方案: -
提取方案: -
建库方案: Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 μl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
测序信息
分子类型: poly(A)+ RNA
库的片段类型: PAIRED
库的链类型: -
测序平台: ILLUMINA
测序仪型号: Illumina HiSeq 2500
链特异性: Unspecific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源 GEN样本编号 GEN数据集编号 系列编号 项目编号 样本编号 样本名称 生物样本编号 样本访问号 实验访问号 释放时间 提交时间 最后更新时间 物种 种族 族裔 年龄 年龄单位 性别 来源名称 组织 细胞类型 细胞亚型 细胞系 疾病 疾病状态 发育阶段 突变/变异 表型 Condition Detail 生长方案 处理方案 提取方案 建库方案 分子类型 库的片段类型 链特异性 库的链类型 加标(Spike-In) 测序方法 测序平台 测序仪型号 细胞数 测序片段数 碱基数 平均测序片段长度_1 平均测序片段长度_2 唯一比对率 多重比对率 覆盖度
文章
A high-density genetic map and multiple environmental tests reveal novel quantitative trait loci and candidate genes for fibre quality and yield in cotton.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik . 2020-09-07 [PMID: 32894321]