PRJCA003821: Genomic basis of rice geographic adaptation to soil nitrogen
概要: Intensive application of inorganic nitrogen underlies marked increase in crop production yet imposes detrimental impact on ecosystems, hence it is crucial for future sustainable agriculture to improve nitrogen-use efficiency (NUE). Here we report the identification of genetic basis of NUE associated with the local soil adaptation of cultivated rice. With a diverse rice germplasm panel collected from different ecogeographic regions, we performed genome-wide association study on tillering response to nitrogen (TRN), the most correlated trait to NUE of rice, and identified OsTCP19 as a modulator of TRN via transcriptionally responding to nitrogen.Using expression profiling by high throughput sequencing, we identified a number of target genes of OsTCP19 demonstrated its enormous potential for rice breeding and environment amelioration through reducing nitrogen application.
| 生长方案: |
- |
| 处理方案: |
- |
| 提取方案: |
Poly (A) RNA from 1 mg total RNA was purified with OligodT beads and then used to generate the cDNA libraries. |
| 建库方案: |
- |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
- |
| 测序平台: |
BGISEQ |
| 测序仪型号: |
BGISEQ-500 |
| 链特异性: |
Unspecific |
| 数据来源 |
GEN样本编号 |
GEN数据集编号 |
系列编号 |
项目编号 |
样本编号 |
样本名称 |
生物样本编号 |
样本访问号 |
实验访问号 |
释放时间 |
提交时间 |
最后更新时间 |
物种 |
种族 |
族裔 |
年龄 |
年龄单位 |
性别 |
来源名称 |
组织 |
细胞类型 |
细胞亚型 |
细胞系 |
疾病 |
疾病状态 |
发育阶段 |
突变/变异 |
表型 |
Condition Detail |
生长方案 |
处理方案 |
提取方案 |
建库方案 |
分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标（Spike-In） |
测序方法 |
测序平台 |
测序仪型号 |
细胞数 |
测序片段数 |
碱基数 |
平均测序片段长度_1 |
平均测序片段长度_2 |
唯一比对率 |
多重比对率 |
覆盖度 |
Genomic basis of geographical adaptation to soil nitrogen in rice.
Nature . 2021-01-06 [PMID:
33408412]
