PRJEB27791: Bulk RNA-seq of of human dermal fibroblasts from up to 66 individuals
概要: Dermal fibroblasts from up to 66 human donors, stimulated with dsRNA (poly I:C) in a time course of 0, 2, 6 hours, profiled using the Smart-seq2 protocol. Donor fibroblast samples were supplied by the Hipsci consortium.
项目整体设计: The innate immune response - the expression programme that is initiated once a pathogen is sensed - is known to be variable among responding cells, as well as to rapidly evolve in the course of mammal evolution. To study the transcriptional divergence and cell-to-cell variability of this response, we stimulated dermal fibroblast cells from two primates (human and macaque) and two rodents (mouse and rat) with dsRNA - a mimic of viral RNA that elicits a rapid innate immune response. Subsequently, we profiled the response using bulk RNA-seq, scRNA-seq and ChIP-seq across the four species and across different time points.
| 生长方案: |
- |
| 处理方案: |
- |
| 提取方案: |
Bulk RNA-seq of of human dermal fibroblasts from up to 66 individuals |
| 建库方案: |
The innate immune response - the expression programme that is initiated once a pathogen is sensed - is known to be variable among responding cells, as well as to rapidly evolve in the course of mammal evolution. To study the transcriptional divergence and cell-to-cell variability of this response, we stimulated dermal fibroblast cells from two primates (human and macaque) and two rodents (mouse and rat) with dsRNA - a mimic of viral RNA that elicits a rapid innate immune response. Subsequently, we profiled the response using bulk RNA-seq, scRNA-seq and ChIP-seq across the four species and across different time points. |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
Forward |
| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina HiSeq 2500 |
| 链特异性: |
Specific |
| 数据来源 |
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细胞系 |
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Condition Detail |
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分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标(Spike-In) |
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平均测序片段长度_1 |
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多重比对率 |
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Gene expression variability across cells and species shapes innate immunity.
Nature . 2018-10-24 [PMID:
30356220]