| Growth Protocol: |
Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 12 days, plants were placed to 15°C for 12 h at the beginning of the night (acclimation; A). Sampling was done 2 h after dawn.; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 12 days, plants were placed to 15°C for 12 h at the beginning of the night (acclimation; A). Two hours after dawn, plants were transfered to cold stress (5°C; the medium and the climate chamber was pre-cooled) for 24 h. Sampling was done 2 h after dawn (12-days-old plants); Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 12 days, plants were placed to 15°C for 12 h at the beginning of the night (acclimation; A). Two hours after dawn, plants were transfered to cold stress (5°C; the medium and the climate chamber was pre-cooled) for 24 h. Sampling was done 2 h after dawn (13-days-old plants); Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 12 days, plants were placed to 15°C for 12 h at the beginning of the night (acclimation; A). Two hours after dawn, plants were transfered to cold stress (5°C; the medium and the climate chamber was pre-cooled) for 24 h. Sampling was done 2 h after dawn (14-days-old plants); Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 12 days, plants were placed to 15°C for 12 h at the beginning of the night (acclimation; A). Two hours after dawn, plants were transfered to cold stress (5°C; the medium and the climate chamber was pre-cooled) for 24 h. Two hours after dawn (14-days-old plants), plants were transfered to control conditions (medium was changed) and left under control conditions for 3 days (recovery). Sampling was done after recovery 2 h after dawn.; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 12 days, plants were placed to 15°C for 12 h at the beginning of the night (acclimation; A). Two hours after dawn, plants were transfered to special thermoregulatory vessels (keeping 20°C of new medium) into pre-cooled chamber (5°C of air) for 24 h. Sampling was done 2 h after dawn (14-days-old plants).; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 12 days, plants were placed to 15°C for 12 h at the beginning of the night (acclimation; A). Two hours after dawn, plants were transfered to special thermoregulatory vessels (keeping 20°C of new medium) into pre-cooled chamber (5°C of air) for 24 h. Two hours after dawn (14-days-old plants), plants were transfered to control conditions (medium was changed) and left under control conditions for 3 days (recovery). Sampling was done after recovery 2 h after dawn.; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 12 days, plants were placed to 15°C for 12 h at the beginning of the night (acclimation; A). Two hours after dawn, plants were transfered to special thermoregulatory vessels (keeping 5°C of new medium) into chamber (20°C of air) for 24 h. Sampling was done 2 h after dawn (14-days-old plants).; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 12 days, plants were placed to 15°C for 12 h at the beginning of the night (acclimation; A). Two hours after dawn, plants were transfered to special thermoregulatory vessels (keeping 5°C of new medium) into chamber (20°C of air) for 24 h. Two hours after dawn (14-days-old plants), plants were transfered to control conditions (medium was changed) and left under control conditions for 3 days (recovery). Sampling was done after recovery 2 h after dawn.; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 13 days 2 h after dawn, plants were transfered to cold stress (5°C; the medium and the climate chamber was pre-cooled) for 24 h. Sampling was done 2 h after dawn (14-days-old plants).; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 13 days 2 h after dawn, plants were transfered to cold stress (5°C; the medium and the climate chamber was pre-cooled) for 24 h. Two hours after dawn (14-days-old plants), plants were transfered to control conditions (medium was changed) and left under control conditions for 3 days (recovery). Sampling was done after recovery 2 h after dawn.; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 13 days 2 h after dawn, plants were transfered to special thermoregulatory vessels (keeping 20°C of new medium) into pre-cooled chamber (5°C of air) for 24 h. Sampling was done 2 h after dawn (14-days-old plants).; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 13 days 2 h after dawn, plants were transfered to special thermoregulatory vessels (keeping 20°C of new medium) into pre-cooled chamber (5°C of air) for 24 h. Two hours after dawn (14-days-old plants), plants were transfered to control conditions (medium was changed) and left under control conditions for 3 days (recovery). Sampling was done after recovery 2 h after dawn.; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 13 days 2 h after dawn, plants were transfered to special thermoregulatory vessels (keeping 5°C of new medium) into chamber (20°C of air) for 24 h. Sampling was done 2 h after dawn (14-days-old plants).; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. After 13 days 2 h after dawn, plants were transfered to special thermoregulatory vessels (keeping 5°C of new medium) into chamber (20°C of air) for 24 h. Two hours after dawn (14-days-old plants), plants were transfered to control conditions (medium was changed) and left under control conditions for 3 days (recovery). Sampling was done after recovery 2 h after dawn.; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. Sampling of 13-days-old plants was done 2 h after dawn.; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. Sampling of 14-days-old plants was done 2 h after dawn.; Plants were grown in Sanyo chambers in Araponics hydroponics (Yoshida), 120 μmol/m2/s, 14/10 h 27/23°C light/dark, 70% RH. Sampling of 17-days-old plants was done 2 h after dawn. |
