生长方案:	X.kitaake was used as wild type. Rice seeds were dehulled, sterilized with 50% bleach for 10 min, and rinsed four to five times with sterile water. All plant growth was in Yoshida’s nutrient solution solidified with gellan gum (Gelzan, Caisson Inc).Growth boxes were kept at 4°C in the dark for 2-3 days until the seeds germinate, then transferred to a percival growth chamber set to 28°C, and consistant light. Rice seedlings were grown in the percival for 1-3 days before harvesting.
处理方案:	null
提取方案:	For RNA isolation, root tips were ground to a fine powder in liquid nitrogen using a mortar and pestle, followed by the addition of 1 ml of RLT buffer to the powdered tissue. RNA was then isolated and purified using the RNeasy Mini Kit (QiagenTM) according to the manufacturer’s protocol.
建库方案:	Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dTTP for non strand specific library or dUTP for strand specific library. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification, with Novogene NGS RNA Library Prep Set. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.

分子类型:	Poly(A)+ RNA
库的片段类型:	PAIRED
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	Illumina NovaSeq 6046; Illumina NovaSeq 6047; Illumina NovaSeq 6048; Illumina NovaSeq 6049; Illumina NovaSeq 6050; Illumina NovaSeq 6051; Illumina NovaSeq 6052; Illumina NovaSeq 6053; Illumina NovaSeq 6054; Illumina NovaSeq 6055; Illumina NovaSeq 6056; Illumina NovaSeq 6057; Illumina NovaSeq 6058; Illumina NovaSeq 6059; Illumina NovaSeq 6060
链特异性:	Unspecific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度