Growth Protocol:	X.kitaake was used as wild type. Rice seeds were dehulled, sterilized with 50% bleach for 10 min, and rinsed four to five times with sterile water. All plant growth was in Yoshida’s nutrient solution solidified with gellan gum (Gelzan, Caisson Inc).Growth boxes were kept at 4°C in the dark for 2-3 days until the seeds germinate, then transferred to a percival growth chamber set to 28°C, and consistant light. Rice seedlings were grown in the percival for 1-3 days before harvesting.
Treatment Protocol:	null
Extract Protocol:	For RNA isolation, root tips were ground to a fine powder in liquid nitrogen using a mortar and pestle, followed by the addition of 1 ml of RLT buffer to the powdered tissue. RNA was then isolated and purified using the RNeasy Mini Kit (QiagenTM) according to the manufacturer’s protocol.
Library Construction Protocol:	Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dTTP for non strand specific library or dUTP for strand specific library. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification, with Novogene NGS RNA Library Prep Set. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.

Molecule Type:	Poly(A)+ RNA
Library Source:
Library Layout:	PAIRED
Library Strand:	-
Platform:	ILLUMINA
Instrument Model:	Illumina NovaSeq 6046; Illumina NovaSeq 6047; Illumina NovaSeq 6048; Illumina NovaSeq 6049; Illumina NovaSeq 6050; Illumina NovaSeq 6051; Illumina NovaSeq 6052; Illumina NovaSeq 6053; Illumina NovaSeq 6054; Illumina NovaSeq 6055; Illumina NovaSeq 6056; Illumina NovaSeq 6057; Illumina NovaSeq 6058; Illumina NovaSeq 6059; Illumina NovaSeq 6060
Strand-Specific:	Unspecific

Data Resource	GEN Sample ID	GEN Dataset ID	Project ID	BioProject ID	Sample ID	Sample Name	BioSample ID	Sample Accession	Experiment Accession	Release Date	Submission Date	Update Date	Species	Race	Ethnicity	Age	Age Unit	Gender	Source Name	Tissue	Cell Type	Cell Subtype	Cell Line	Disease	Disease State	Development Stage	Mutation	Phenotype	Condition Detail	Growth Protocol	Treatment Protocol	Extract Protocol	Library Construction Protocol	Molecule Type	Library Layout	Strand-Specific	Library Strand	Spike-In	Strategy	Platform	Instrument Model	Cell Number	Reads Number	Gbases	AvgSpotLen1	AvgSpotLen2	Uniq Mapping Rate	Multiple Mapping Rate	Coverage Rate