Gene Expression
Nebulas
Gene Expression Nebulas概要: Find the casual relationship between gene expression network and cellular phenotype at single cell resolution. We collected donated human pre-implatation embryos; and the embryonic stem cells derived from them; isolate individual cells; prepared single cell cDNAs; and sequenced them by HiSeq2000. Then we analyzed the expression of known RefSeq genes.
项目整体设计: Development and applications of single-cell transcriptome analysis. Nat Meth 8; S6-S11.
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| 生长方案: | Donated human preplantation embryos were dissociated into individual blastmeres and were separately amplied to set up single cell cDNA libraries. |
| 处理方案: | - |
| 提取方案: | Amplied single cell cDNAs were further amplied for another ten cycles of PCR. Then it was sonicated into 200~500bp fragments ,then the standard TruSeq DNA library preparation kit was used following the manufacturer suggestion protocol |
| 建库方案: | Amplied single cell cDNAs were further amplied for another ten cycles of PCR. Then it was sonicated into 200~500bp fragments ,then the standard TruSeq DNA library preparation kit was used following the manufacturer suggestion protocol |
| 分子类型: | poly(A)+ RNA |
| 库的片段类型: | SINGLE |
| 库的链类型: | - |
| 测序平台: | ILLUMINA |
| 测序仪型号: | Illumina HiSeq 2000 |
| 链特异性: | Unspecific |
| 数据来源 | GEN样本编号 | GEN数据集编号 | 系列编号 | 项目编号 | 样本编号 | 样本名称 | 生物样本编号 | 样本访问号 | 实验访问号 | 释放时间 | 提交时间 | 最后更新时间 | 物种 | 种族 | 族裔 | 年龄 | 年龄单位 | 性别 | 来源名称 | 组织 | 细胞类型 | 细胞亚型 | 细胞系 | 疾病 | 疾病状态 | 发育阶段 | 突变/变异 | 表型 | Condition Detail | 生长方案 | 处理方案 | 提取方案 | 建库方案 | 分子类型 | 库的片段类型 | 链特异性 | 库的链类型 | 加标（Spike-In） | 测序方法 | 测序平台 | 测序仪型号 | 细胞数 | 测序片段数 | 碱基数 | 平均测序片段长度_1 | 平均测序片段长度_2 | 唯一比对率 | 多重比对率 | 覆盖度 |
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