生长方案:	All soybean (Glycine max (L.) Merrill) lines were grown in growth chambers with temperature set at 25°C day/ 23°C night, the humidity at 50% and 16 hour days at up to 1,000 µmol of supplemental lighting. The plants were watered, fertilized and managed for pests and disease as needed. Seeds at the S6 stage of seed maturation were carefully selected based on seed weight and color, and were harvested for RNA preparation.
处理方案:	The oil composition of soybean seeds was determined with the Agilent 7890A GC with S/Sl injection and FID detection at the USDA-ARS Plant Genetic Resources Conservation Unit (PGRCU) in Griffin, Georgia. Three single seeds per soybean line were measured with two replications each. The oil content of soybean seeds was determined with the MQC Benchtop NMR Analyser, Oxford Instruments. Six g of seeds per line were measured with two biological and three technical replicates.
提取方案:	Samples were ground in liquid nitrogen. Then rna was separated out with phenol chloroform and then purified and precipitated out using a Qiagen RNeasy Kit
建库方案:	RNA-seq libraries were constructed and sequenced at Expression Analysis, Inc., Durham NC (www.ExpressionAnalysis.com). RNA-seq libraries were prepared with the TruSeqTM RNA Sample Preparation Kit v2 from Illumina, Inc., San Diego, CA, and 100 bp paired-end reads were generated on the Illumina HiSeq 2000 platform.

分子类型:	poly(A)+ RNA
库的片段类型:	PAIRED
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 2000
链特异性:	Unspecific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度