PRJNA274140: Human Embryo Single Cell Transcriptome Analysis
概要: We sorted 2PN stage human embryos by predicted viability using a noninvasive measurement we previously developed. We then performed RNA-seq on each embryo (which was a single cell) and looked for differences in expression between viable and nonviable embryos.
项目整体设计: We sequenced the transcriptomes of 22 human zygotes (2PN stage) embryos; of which 11 were predicted nonviable and 11 were predicted viable. We excluded 5 which had no siblings in this data set. From the remaining 17 embryos; we analyzed the set of genes that was differentially expressed between those predicted viable and those predicted nonviable.
| 测序方法: |
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| 物种: |
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| 组织: |
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| 健康状况: |
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| 细胞类型: |
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| 发育阶段: |
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| 生长方案: |
- |
| 处理方案: |
- |
| 提取方案: |
SMARTer Ultra Low RNA kit (Clontech) |
| 建库方案: |
NEBNext DNA Sample Prep Master Mix Set (New England Biolabs) |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
- |
| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina HiSeq 2000 |
| 链特异性: |
Unspecific |
| 数据来源 |
GEN样本编号 |
GEN数据集编号 |
系列编号 |
项目编号 |
样本编号 |
样本名称 |
生物样本编号 |
样本访问号 |
实验访问号 |
释放时间 |
提交时间 |
最后更新时间 |
物种 |
种族 |
族裔 |
年龄 |
年龄单位 |
性别 |
来源名称 |
组织 |
细胞类型 |
细胞亚型 |
细胞系 |
疾病 |
疾病状态 |
发育阶段 |
突变/变异 |
表型 |
Condition Detail |
生长方案 |
处理方案 |
提取方案 |
建库方案 |
分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标（Spike-In） |
测序方法 |
测序平台 |
测序仪型号 |
细胞数 |
测序片段数 |
碱基数 |
平均测序片段长度_1 |
平均测序片段长度_2 |
唯一比对率 |
多重比对率 |
覆盖度 |
Human oocyte developmental potential is predicted by mechanical properties within hours after fertilization.
Nature communications . 2016-02-24 [PMID:
26904963]
