生长方案:	The seedlings of the NILs were grown in an environment controlled greenhouse. The experimental design was a randomized complete block with four replications. For each NIL, four seeds per replicate for each of the four sampling times (0, 8, 24, 48 hrs after aphid infestation) were planted in 4-cm diameter x 15-cm deep plastic conetainers in a USDA greenhouse at Ohio Agricultural Research and Development Center (OARDC), Wooster, OH and the best two seedlings per conetainer were kept after germination. The conetainers were arranged in a rack with 8-cm x 12-cm spacing on a greenhouse bench. The greenhouse was maintained at approximately 26/22˚C day/night temperatures with 15 h light daily. The biotype 2 soybean aphids used in this study was from a growth chamber colony maintained at OARDC, Wooster, OH 34 . At the V2 growth stage, each soybean seedling was manually infested with 15 adult soybean aphids by placing the aphids on the top leaves of the seedling. For the 0 hr samples, just before infestation of seedlings with soybean aphids, the youngest unfolded leaves and the tips of two seedlings representing each replicate of each NIL were collected in a 2 ml tube and were immediately frozen in liquid nitrogen. The samples for 8, 24, and 48 h after infestation, were collected in the same manner, except that the aphids were gently removed from the tissue by using a soft paint brush before tissue collection. The aphids continued to walk away from the resistant seedlings within 12 to 24 hours of placement on the seedlings, so more aphids were added to these seedlings every 12 hrs to have at least 15 aphids per seedling at all times.
处理方案:	Aphid infestation
提取方案:	Total RNA were extracted using Trizol reagent according to manufacturer's instruction
建库方案:	Complementary DNA libraries were built according to the manusfacturer’s instruction (mRNA sequencing, Sample preparation guide; Illumina, San Diego, CA) using 10 µg of RNA. Each library was labeled with a 4 nucleotide specific barcode ACGT, CGTT, GTAT and sequenced using a HiSeq 2000 (Illumina, San Diego, CA)

分子类型:	poly(A)+ RNA
库的片段类型:	SINGLE
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 2000
链特异性:	Unspecific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度