生长方案:	Undifferentiated feeder-free iPSCs generated from Yoruba LCLs were grown in E8 medium (Life Tech) (G. Chen et al. 2011) on Matrigel-coated tissue culture plates with daily media feeding at 37 °C with 5% (vol/col) CO2. For standard maintenance, cells were split every 3-4 days using cell release solution (0.5 mM EDTA and NaCl in PBS) at the confluence of roughly 80%. For the single cell suspension, iPSCs were individualized by Accutase Cell Detachment Solution (BD) for 5-7 minutes at 37 °C and washed twice with E8 media immediately before each experiment. Cell viability and cell counts were then measured by the Automated Cell Counter (Bio-Rad) to generate resuspension densities of 2.5 X 105 cells/mL in E8 medium for C1 cell capture.; Undifferentiated feeder-free iPSCs generated from Yoruba LCLs were grown in E8 medium (Life Tech) (G. Chen et al. 2011) on Matrigel-coated tissue culture plates with daily media feeding at 37 °C with 5% (vol/col) CO2. For standard maintenance, cell were split every 3-4 days using cell release solution (0.5 mM EDTA and NaCl in PBS) at the confluence of roughly 80%. For the single cell suspension, iPSCs were individualized by Accutase Cell Detachment Solution (BD) for 5-7 minutes at 37 °C and washed twice with E8 media immediately before each experiment. Cell viability and cell counts were then measured by the Automated Cell Counter (Bio-Rad) to generate resuspension densities of 2.5 X 105 cell/mL in E8 medium for C1 cell capture.
处理方案:	-
提取方案:	Single cell loading and capture was performed following the Fluidigm manual; A bulk sample, a 40 ul aliquot of ~10,000 cell, was collected in parallel with each C1 chip using the same reaction mixes following the C1 protocol of ""Tube Controls with Purified RNA
建库方案:	For sequencing library preparation, fragmentation and isolation of 5^ fragments were performed according to the UMI protocol (Islam et al. 2014). Instead of using commercial available Tn5 transposase, Tn5 protein stock was freshly purified in house using the IMPACT system (pTXB1, NEB) following the protocol previously described (Picelli et al. 2014). The activity of Tn5 was tested and shown to be comparable with the EZ-Tn5-Transposase (Epicentre). Importantly, all the libraries in this study were generated using the same batch of Tn5 protein purification. For each of the bulk samples, two libraries were generated using two different indices in order to get sufficient material.

分子类型:	poly(A)+ RNA
库的片段类型:	SINGLE
库的链类型:	Reverse
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 2500
链特异性:	Specific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度