生长方案:	Cells were resuspended in DMEM/F12 with addition of B27 supplement (1:1000), N2 supplement (1:100), 20 ng/ml FGF-2, 20 ng/ml EGF, 1.6 g/l glucose, 20 ?g/ml insulin and 5 ng/ml BDNF. 10 ?M rock inhibitor (Y-27632, Stemgent 04-0012) was added to increase cell viability. The cells were then plated into dishes coated with poly-L-ornithine (0.01%, Sigma P4957), laminin (5 ?g/ml, Invitrogen 23017-015) and fibronectin (1μg/ml, Corning, 354008,). Medium was changed 12 hr after plating and the cells were passaged using 0.25% trypsin within 3 d and replated to obtain a monolayer of cells at a density of 4–5 × 104 cells/cm2. Half of the media was changed every 2–3 d to allow culture conditioning."
处理方案:	-
提取方案:	RNA was extracted after single cell capture using C1 single cell auto prep system, with manufacturer protocol.
建库方案:	RNA libraries were prepared for sequencing using with Illumina NEXTERA XT kit

分子类型:	poly(A)+ RNA
库的片段类型:	SINGLE
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 2000
链特异性:	Unspecific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度