概要: Abstract from Vermillion et al: During vertebrate development, progenitor cells give rise to tissues and organs through a complex choreography that commences at gastrulation. A hallmark event of gastrulation is the formation of the primitive streak, a linear assembly of cells along the anterior-posterior (AP) axis of the developing organism. To examine the primitive streak at a single-cell resolution, we measured the transcriptomes of individual chick cells from the streak or the surrounding tissue (the rest of the area pellucida) in Hamburger-Hamilton stage 4 embryos. The single-cell transcriptomes were then ordered by the statistical method Wave-Crest to deduce both the relative position along the AP axis and the prospective lineage of single cells. The ordered transcriptomes reveal intricate patterns of gene expression along the primitive streak.
项目整体设计: Examination of single-cells of stage 4 chicken embryos.
Hamburger-Hamilton Stage 4 chicken embryos were collected in Ringer's solution. The primitive streak was dissected out using a fine tungsten needle, and the remaining surrounding tissue (rest of the area pellucida) was collected separately.
建库方案:
iluted single-cell cDNA libraries were fragmented and amplified using the Nextera XT DNA Sample Preparation Kit and the Nextera XT DNA Sample Preparation Index Kit (Illumina).scRNA-seq with ~40x multiplexing
测序信息
分子类型:
poly(A)+ RNA
库的片段类型:
SINGLE
库的链类型:
-
测序平台:
ILLUMINA
测序仪型号:
Illumina HiSeq 2500
链特异性:
Unspecific
样本
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样本描述:
生物条件:
实验变量:
方案:
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质量评估:
数据来源
GEN样本编号
GEN数据集编号
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样本名称
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物种
种族
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组织
细胞类型
细胞亚型
细胞系
疾病
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发育阶段
突变/变异
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Condition Detail
生长方案
处理方案
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建库方案
分子类型
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库的链类型
加标（Spike-In）
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碱基数
平均测序片段长度_1
平均测序片段长度_2
唯一比对率
多重比对率
覆盖度
文章
Spatial patterns of gene expression are unveiled in the chick primitive streak by ordering single-cell transcriptomes.
