生长方案:	Chlorella vulgaris (SAG 211-12) was obtained from the collection of algal cultures at the University of Göttingen, Germany. The inoculum was grown in Bold’s Basal medium consisting of (per liter): 0.25 g NaNO3, 0.075 g MgSO4.7H2O, 0.075 g K2HPO4, 0.175 g KH2PO4, 0.025 g NaCl, 0.025 g CaCl2.2H2O, 8.82 mg ZnSO4.7H2O, 0.44 mg MnCl2.4H2O, 0.71 mg MoO3, 1.57 mg CuSO4.5H2O, 0.49 mg CoCl2.6H2O, 11.42 mg H3BO3, 50 mg EDTA, 31 mg KOH, 4.98 mg feSO4.7H2O. Cultures were maintained at 25◦C under continuous illumination by four fluorescent lamps
处理方案:	250 µl culture of C. vulgaris with the cell count of 4×10e8 cells/ml was spread on solid Bold’s basal media and then exposed to UV irradiation (254 nm) with an intensity of 2.9 ×10-2 W/cm2 for 0.5 to 10 minutes at a distance of 15 cm. UVX Digital Radiometer (UVP, USA) was used to measure the UV-light intensity. The UV-irradiated plates were kept in dark for 24 h to prevent light-induced repair. The plates were then maintained under normal light for 2 weeks. Single colonies appearing on plates were selected and transferred individually into 2 ml Bold’s basal medium. After 10 days of culturing, the cell densities were determined by measuring OD at 680 nm.
提取方案:	Total RNA was extracted from 14-days old stationary phase reached (OD680=5) 5 ml cell culture using TRIzol reagent (Invitrogen, USA) according to the manufacturer’s instructions. Briefly, after quality and quantity measurement using 2100 BioAnalyzer (Agilent, USA), it was treated with RNase-free DNase I (Thermo Scientific, USA) at a concentration of 1 U/µg to remove residual genomic DNA. Then, RNA-seq library preparation was performed using TruSeq mRNA Sample Preparation Kit (Illumina, USA) according to the manufacturer’s instructions. mRNAs were purified from the 1 µg of total RNA using oligo (dT) magnetic beads and fragmented using fragmentation buffer. Afterthat, the cleaved short RNA fragments were used for first-strand cDNA synthesis using first strand synthesis mix, and the second strand was synthesized using second strand marking master mix. The double strand cDNAs were purified with AMPure XP beads (Beckman Coulter, USA) and eluted with resuspension buffer followed by 3’end adenine nucleotide addition. Finally, sequencing adaptors were ligated to the fragments and cDNA fragments were enriched by PCR amplification.
建库方案:	Enriched cDNA libraries were used for cluster generation and sequencing. 75x2 paired-end sequencing of three cDNA libraries (WT, UV-715 and EMS-25) with two biological replicates were performed using the Illumina MiSeq sequencing platform (Illumina, USA). All sequence data are PE 2x75 bp. Image processing, base calling, and quality calue calculation were performed by the Illumina data processing pipeline (v1.5). High quality reads were saved in FASTQ format.

分子类型:	polyA(+) RNA
库的片段类型:	PAIRED
库的链类型:	-; Reverse
测序平台:	ILLUMINA
测序仪型号:	Illumina MiSeq
链特异性:	Unspecific; Specific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度