概要: Sperm cells of seed plants have lost their motility and are transported by the vegetative pollen tube cell for fertilization. The extent to which sperm cells regulate their own transportation is a long-standing debate. By using the novel Arabidopsis double mutant drop1 drop2, we demonstrate here that sperm cells are only passive cargo and that the vegetative tube cell as a vehicle controls the entire journey.
项目整体设计: The semi-in-vivo pollen tubes of wild type (WT) and drop mutant using single-cell RNA sequencing
pollen tubes were growed on the solid pollen germination medium (SPGM), which10% sucrose, 0.01% boric acid, 5 mM CaCl2, 5 mM KCl, 1 mM MgSO4 and pH=7.5.
处理方案:
About 500-600 double mutant pollen grains were hand-picked under a stereoscopic microscope and pollinated on a single stigma (emasculated for 36 hours) and cut together with the style. When the SIV PTs grew about 300-400 μm outside of the style, they were cut from the end of the style with tiny scissors (VANNAS, 8.5 cm, STR) under a stereo microscope.
提取方案:
Poly(A)+ RNA
建库方案:
The lysed solution was used to generate the cDNA libraries. The cDNA libraries follow the Smart-seq2 protocol.
测序信息
分子类型:
poly(A)+ RNA
库的片段类型:
PAIRED
库的链类型:
-
测序平台:
ILLUMINA
测序仪型号:
Illumina HiSeq 4000
链特异性:
Unspecific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源
GEN样本编号
GEN数据集编号
系列编号
项目编号
样本编号
样本名称
生物样本编号
样本访问号
实验访问号
释放时间
提交时间
最后更新时间
物种
种族
族裔
年龄
年龄单位
性别
来源名称
组织
细胞类型
细胞亚型
细胞系
疾病
疾病状态
发育阶段
突变/变异
表型
Condition Detail
生长方案
处理方案
提取方案
建库方案
分子类型
库的片段类型
链特异性
库的链类型
加标（Spike-In）
测序方法
测序平台
测序仪型号
细胞数
测序片段数
碱基数
平均测序片段长度_1
平均测序片段长度_2
唯一比对率
多重比对率
覆盖度
文章
Sperm cells are passive cargo of the pollen tube in plant fertilization.
