Gene Expression Nebulas
基于标准化流程分析的转录图谱综合数据库

Gene Expression Nebulas

多物种转录图谱整合数据库

PRJNA386172: Co-overexpression of the constitutively active form of OsbZIP46 and ABA-activated protein kinase SAPK6 improves drought and temperature stress resistance in rice

来源: NCBI / GSE98775
提交时间: May 10, 2017
释放时间: Aug 10, 2017
最后更新时间: Jul 25, 2021

概要: Drought is one of the major abiotic stresses threatening rice (Oryza sativa) production worldwide. Drought resistance is controlled by multiple genes, and therefore, a multi-gene genetic engineering strategy is theoretically useful for improving drought resistance. However, the experimental evidence for such a strategy is still lacking. In this study, a few drought-responsive genes from rice were assembled by a multiple-round site-specific assembly (MISSA) system, and the constructs were introduced into the rice cultivar KY131 via Agrobacterium-mediated transformation. The transgenic lines of the multi-gene and corresponding single-gene constructs were pre-evaluated for drought resistance. We found that the co-overexpression of two genes, encoding a constitutively active form of a bZIP transcription factor (OsbZIP46CA1) and a protein kinase (SAPK6) involved in the abscisic acid (ABA) signaling pathway, showed significantly enhanced drought resistance compared with the single-gene transgenic lines and the negative transgenic plants. Single-copy lines of this bi-gene combination (named XL22) and the corresponding single-gene lines were further evaluated for drought resistance in the field using agronomical traits. The results showed that XL22 exhibited greater yield, biomass, spikelet number, and grain number under moderate drought stress conditions. The seedling survival rate of XL22 and the single-gene overexpressors after drought stress treatment also supported the drought resistance results. Furthermore, expression profiling by RNA-Seq revealed that many genes involved in the stress response were specifically up-regulated in the drought-treated XL22 lines and some of the stress-related genes activated in CA1-OE and SAPK6-OE were distinct, which could partially explain the different performances of these lines with respect to drought resistance. In addition, the XL22 seedlings showed improved tolerance to heat and cold stresses. Our results demonstrate that the multi-gene assembly in an appropriate combination may be a promising approach in the genetic improvement of drought resistance.

项目整体设计: Transcriptome analysis of XL22, CA1-OE, and SAPK6-OE plants under both severe drought stress and normal growth conditions.

GEN 数据集:
GEND000530
测序方法:
物种:
组织:
健康状况:
细胞类型:
细胞系:
发育阶段:
方案
生长方案: plants were grown in a paddy field facilitated with a movable rain-off shelter during the summer of 2016 in Wuhan, Hubei Province, China. Ten plants for each family were planted in two rows (one plot) with a planting density similar to real agricultural fields.; plants were grown in a paddy field facilitated with a movable rain-off shelter during the summer of 2017 in Wuhan, Hubei Province, China. Ten plants for each family were planted in two rows (one plot) with a planting density similar to real agricultural fields.; plants were grown in a paddy field facilitated with a movable rain-off shelter during the summer of 2018 in Wuhan, Hubei Province, China. Ten plants for each family were planted in two rows (one plot) with a planting density similar to real agricultural fields.; plants were grown in a paddy field facilitated with a movable rain-off shelter during the summer of 2019 in Wuhan, Hubei Province, China. Ten plants for each family were planted in two rows (one plot) with a planting density similar to real agricultural fields.; plants were grown in a paddy field facilitated with a movable rain-off shelter during the summer of 2020 in Wuhan, Hubei Province, China. Ten plants for each family were planted in two rows (one plot) with a planting density similar to real agricultural fields.; plants were grown in a paddy field facilitated with a movable rain-off shelter during the summer of 2021 in Wuhan, Hubei Province, China. Ten plants for each family were planted in two rows (one plot) with a planting density similar to real agricultural fields.; plants were grown in a paddy field facilitated with a movable rain-off shelter during the summer of 2022 in Wuhan, Hubei Province, China. Ten plants for each family were planted in two rows (one plot) with a planting density similar to real agricultural fields.; plants were grown in a paddy field facilitated with a movable rain-off shelter during the summer of 2023 in Wuhan, Hubei Province, China. Ten plants for each family were planted in two rows (one plot) with a planting density similar to real agricultural fields.; plants were grown in a paddy field facilitated with a movable rain-off shelter during the summer of 2024 in Wuhan, Hubei Province, China. Ten plants for each family were planted in two rows (one plot) with a planting density similar to real agricultural fields.; plants were grown in a paddy field facilitated with a movable rain-off shelter during the summer of 2025 in Wuhan, Hubei Province, China. Ten plants for each family were planted in two rows (one plot) with a planting density similar to real agricultural fields.; plants were grown in a paddy field facilitated with a movable rain-off shelter during the summer of 2026 in Wuhan, Hubei Province, China. Ten plants for each family were planted in two rows (one plot) with a planting density similar to real agricultural fields.; plants were grown in a paddy field facilitated with a movable rain-off shelter during the summer of 2027 in Wuhan, Hubei Province, China. Ten plants for each family were planted in two rows (one plot) with a planting density similar to real agricultural fields.
处理方案: Drought stress was applied by stopping watering at the booting stage (about 2 weeks before flowering) in the field, and drought stress was considered to take place when the mean TDR (Time Domain Reflectometry) value of a block was less than 25; Drought stress was applied by stopping watering at the booting stage (about 2 weeks before flowering) in the field, and drought stress was considered to take place when the mean TDR (Time Domain Reflectometry) value of a block was less than 26; Drought stress was applied by stopping watering at the booting stage (about 2 weeks before flowering) in the field, and drought stress was considered to take place when the mean TDR (Time Domain Reflectometry) value of a block was less than 27; Drought stress was applied by stopping watering at the booting stage (about 2 weeks before flowering) in the field, and drought stress was considered to take place when the mean TDR (Time Domain Reflectometry) value of a block was less than 28; Drought stress was applied by stopping watering at the booting stage (about 2 weeks before flowering) in the field, and drought stress was considered to take place when the mean TDR (Time Domain Reflectometry) value of a block was less than 29; Drought stress was applied by stopping watering at the booting stage (about 2 weeks before flowering) in the field, and drought stress was considered to take place when the mean TDR (Time Domain Reflectometry) value of a block was less than 30; Drought stress was applied by stopping watering at the booting stage (about 2 weeks before flowering) in the field, and drought stress was considered to take place when the mean TDR (Time Domain Reflectometry) value of a block was less than 31; Drought stress was applied by stopping watering at the booting stage (about 2 weeks before flowering) in the field, and drought stress was considered to take place when the mean TDR (Time Domain Reflectometry) value of a block was less than 32; Drought stress was applied by stopping watering at the booting stage (about 2 weeks before flowering) in the field, and drought stress was considered to take place when the mean TDR (Time Domain Reflectometry) value of a block was less than 33; Drought stress was applied by stopping watering at the booting stage (about 2 weeks before flowering) in the field, and drought stress was considered to take place when the mean TDR (Time Domain Reflectometry) value of a block was less than 34; Drought stress was applied by stopping watering at the booting stage (about 2 weeks before flowering) in the field, and drought stress was considered to take place when the mean TDR (Time Domain Reflectometry) value of a block was less than 35; Drought stress was applied by stopping watering at the booting stage (about 2 weeks before flowering) in the field, and drought stress was considered to take place when the mean TDR (Time Domain Reflectometry) value of a block was less than 36
提取方案: RNA for the RNA-Seq analysis was extracted using TRIzol® reagent (AmbionTM, Lot No. 15596018) according to the manufacturer’s protocol.
建库方案: Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations.
测序信息
分子类型: rRNA- RNA
库的片段类型: SINGLE
库的链类型: Forward
测序平台: ILLUMINA
测序仪型号: Illumina HiSeq 2000
链特异性: Specific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源 GEN样本编号 GEN数据集编号 系列编号 项目编号 样本编号 样本名称 生物样本编号 样本访问号 实验访问号 释放时间 提交时间 最后更新时间 物种 种族 族裔 年龄 年龄单位 性别 来源名称 组织 细胞类型 细胞亚型 细胞系 疾病 疾病状态 发育阶段 突变/变异 表型 Condition Detail 生长方案 处理方案 提取方案 建库方案 分子类型 库的片段类型 链特异性 库的链类型 加标(Spike-In) 测序方法 测序平台 测序仪型号 细胞数 测序片段数 碱基数 平均测序片段长度_1 平均测序片段长度_2 唯一比对率 多重比对率 覆盖度